AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS STEM SEGMENTS AND REGENERATION OF TRANSGENIC PLANTS

被引：136
|
作者：
MOORE, GA
JACONO, CC
NEIDIGH, JL
LAWRENCE, SD
CLINE, K
机构：
[1] Fruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, 32611, FL
来源：
PLANT CELL REPORTS | 1992年 / 11卷 / 5-6期
关键词：
CARRIZO CITRANGE; KEY LIME (C-AURANTIFOLIA); GENETIC TRANSFORMATION;
D O I：
10.1007/BF00235073
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker beta-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (less-than-or-equal-to 5%) of the shoots regenerated in the presence of 100-mu-g/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.
引用
收藏
页码：238 / 242
页数：5
相关论文
共 50 条
  • [21] Agrobacterium-mediated transformation, selection and regeneration of Vanilla pompona
    Edmond, Vovener de Verlands
    Moon, Pamela A.
    Bremgartner, Matthew
    Wu, Xingbo
    Bassil, Elias
    PLANT CELL TISSUE AND ORGAN CULTURE, 2024, 158 (02)
  • [22] Regeneration and Agrobacterium-mediated genetic transformation in Dianthus chinensis
    Zhang, Xiaoni
    Wu, Quanshu
    Lin, Shengnan
    Zhang, Zhen
    Wang, Zehao
    Wang, Qijian
    Yan, Xiuli
    Bendahmane, Mohammed
    Bao, Manzhu
    Fu, Xiaopeng
    SCIENTIA HORTICULTURAE, 2021, 287
  • [23] Agrobacterium-mediated transformation and regeneration of Freesia x hybrida
    Uwagaki, Yohei
    Matsuda, Eriko
    Komaki, Masako
    Murahama, Minoru
    Otani, Motoyasu
    Nishizawa, Naoko
    Hamada, Tatsuro
    PLANT BIOTECHNOLOGY, 2015, 32 (02) : 165 - 168
  • [24] Early events in Agrobacterium-mediated genetic transformation of citrus explants
    Peña, L
    Pérez, RM
    Cervera, M
    Juárez, JA
    Navarro, L
    ANNALS OF BOTANY, 2004, 94 (01) : 67 - 74
  • [25] Regeneration of transgenic plants by Agrobacterium-mediated transformation of Quercus ilex L. somatic embryos with the gene CsTL1
    V. Cano
    M. T. Martínez
    M. C. San José
    J. L. Couselo
    E. Varas
    L. Bouza-Morcillo
    M. Toribio
    E. Corredoira
    New Forests, 2020, 51 : 1003 - 1021
  • [26] Agrobacterium-mediated transformation of citrus using two binary vectors
    Soneji, Jaya R.
    Chen, Chunxian
    Rao, M. Nageswara
    Huang, Shu
    Choi, Young A.
    Gmitter, Fred G., Jr.
    PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF TEMPERATE FRUIT CROPS AND TROPICAL SPECIES, 2007, (738): : 261 - +
  • [27] Efficient production of transgenic melon via Agrobacterium-mediated transformation
    Bezirganoglu, I.
    Hwang, S. Y.
    Shaw, J. F.
    Fang, T. J.
    GENETICS AND MOLECULAR RESEARCH, 2014, 13 (02) : 3218 - 3227
  • [28] Regeneration of transgenic plants by Agrobacterium-mediated transformation of Quercus ilex L. somatic embryos with the gene CsTL1
    Cano, V.
    Martinez, M. T.
    San Jose, M. C.
    Couselo, J. L.
    Varas, E.
    Bouza-Morcillo, L.
    Toribio, M.
    Corredoira, E.
    NEW FORESTS, 2020, 51 (06) : 1003 - 1021
  • [29] Agrobacterium-mediated transformation of Asparagus officinalis L.: molecular and genetic analysis of transgenic plants
    A. Limanton-Grevet
    M. Jullien
    Molecular Breeding, 2001, 7 : 141 - 150
  • [30] Agrobacterium-mediated transformation of Asparagus officinalis L.:: molecular and genetic analysis of transgenic plants
    Limanton-Grevet, A
    Jullien, M
    MOLECULAR BREEDING, 2001, 7 (02) : 141 - 150
12345