AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS STEM SEGMENTS AND REGENERATION OF TRANSGENIC PLANTS

被引：136
|
作者：
MOORE, GA
JACONO, CC
NEIDIGH, JL
LAWRENCE, SD
CLINE, K
机构：
[1] Fruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, 32611, FL
来源：
PLANT CELL REPORTS | 1992年 / 11卷 / 5-6期
关键词：
CARRIZO CITRANGE; KEY LIME (C-AURANTIFOLIA); GENETIC TRANSFORMATION;
D O I：
10.1007/BF00235073
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker beta-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (less-than-or-equal-to 5%) of the shoots regenerated in the presence of 100-mu-g/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.
引用
收藏
页码：238 / 242
页数：5
相关论文
共 50 条
  • [1] AGROBACTERIUM-MEDIATED TRANSFORMATION OF SWEET ORANGE AND REGENERATION OF TRANSGENIC PLANTS
    PENA, L
    CERVERA, M
    JUAREZ, J
    NAVARRO, A
    PINA, JA
    DURANVILA, N
    NAVARRO, L
    PLANT CELL REPORTS, 1995, 14 (10) : 616 - 619
  • [2] HIGH-EFFICIENCY AGROBACTERIUM-MEDIATED TRANSFORMATION AND REGENERATION OF CITRUS
    PENA, L
    CERVERA, M
    JUAREZ, J
    ORTEGA, C
    PINA, JA
    DURANVILA, N
    NAVARRO, L
    PLANT SCIENCE, 1995, 104 (02) : 183 - 191
  • [3] Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf segments as explants in Valencia sweet orange
    Ehsan Ullah Khan
    Xing-Zheng Fu
    Ji-Hong Liu
    Plant Cell, Tissue and Organ Culture (PCTOC), 2012, 109 : 383 - 390
  • [4] Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf segments as explants in Valencia sweet orange
    Khan, Ehsan Ullah
    Fu, Xing-Zheng
    Liu, Ji-Hong
    PLANT CELL TISSUE AND ORGAN CULTURE, 2012, 109 (02) : 383 - 390
  • [5] Transgenic patchouli plants produced by Agrobacterium-mediated transformation
    Sugimura, Y
    Kadotani, N
    Ueda, Y
    Shima, K
    Kitajima, S
    Furusawa, T
    Ikegami, M
    PLANT CELL TISSUE AND ORGAN CULTURE, 2005, 82 (03) : 251 - 257
  • [6] Transgenic patchouli plants produced by Agrobacterium-mediated transformation
    Yukio Sugimura
    Naoto Kadotani
    Yoshiko Ueda
    Kaori Shima
    Sakihito Kitajima
    Toshiharu Furusawa
    Masato Ikegami
    Plant Cell, Tissue and Organ Culture, 2005, 82 : 251 - 257
  • [7] Agrobacterium-mediated transformation and regeneration of transgenic 'Chancellor' wine grape plants expressing the tfdA gene
    Mulwa, R. M. S.
    Norton, M. A.
    Farrand, S. K.
    Skirvin, R. M.
    VITIS, 2007, 46 (03) : 110 - 115
  • [8] Regeneration of transgenic plants by Agrobacterium-mediated transformation of somatic embryos of juvenile and mature Quercus robur
    N. Vidal
    R. Mallón
    S. Valladares
    A. M. Meijomín
    A. M. Vieitez
    Plant Cell Reports, 2010, 29 : 1411 - 1422
  • [9] Regeneration of transgenic plants by Agrobacterium-mediated transformation of somatic embryos of juvenile and mature Quercus robur
    Vidal, N.
    Mallon, R.
    Valladares, S.
    Meijomin, A. M.
    Vieitez, A. M.
    PLANT CELL REPORTS, 2010, 29 (12) : 1411 - 1422
  • [10] Transgenic zoysiagrass (Zoysia japonica) plants obtained by Agrobacterium-mediated transformation
    Yaxin Ge
    Tina Norton
    Zeng-Yu Wang
    Plant Cell Reports, 2006, 25 : 792 - 798
12345