PROTEOLYTIC PROCESSING OF THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE PROTEIN-1 PRODUCES A MEMBRANE-BOUND FRAGMENT CONTAINING 2 EPIDERMAL GROWTH FACTOR-LIKE DOMAINS

被引：241
作者：
BLACKMAN, MJ
LING, IT
NICHOLLS, SC
HOLDER, AA
机构：
[1] NATL INST MED RES,DIV PARASITOL,MILL HILL,LONDON NW7 1AA,ENGLAND
[2] WELLCOME RES LABS,BECKENHAM BR3 3BS,KENT,ENGLAND
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1991年 / 49卷 / 01期
基金：
英国医学研究理事会;
关键词：
MALARIA; PLASMODIUM-FALCIPARUM; MEROZOITE SURFACE PROTEIN-1; EPIDERMAL GROWTH FACTOR;
D O I：
10.1016/0166-6851(91)90127-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The amino-terminal sequence has been obtained for 2 fragments of the Plasmodium falciparum T9/94 merozoite surface protein precursor (PfMSP1) and these have been compared with the sequence predicted from the gene. These data define the position of these fragments in the precursor and indicate that the C-terminal sequence which is carried into the red cell during invasion consists of 2 epidermal growth factor (EGF)-like domains. A homologous cleavage sequence and domain structure can be identified in the MSP1 molecules of other malarial species. In addition the results suggest that the smaller fragment is not N-glycosylated.
引用
收藏
页码：29 / 34
页数：6
相关论文
共 26 条
[1]  
AITKEN A, 1989, PROTEIN SEQUENCING P, P43
[2]   STRUCTURE AND FUNCTION OF EPIDERMAL GROWTH FACTOR-LIKE REGIONS IN PROTEINS [J].
APPELLA, E ;
WEBER, IT ;
BLASI, F .
FEBS LETTERS, 1988, 231 (01) :1-4
[3]   PROCESSING OF THE PLASMODIUM-FALCIPARUM MAJOR MEROZOITE SURFACE PROTEIN-1 - IDENTIFICATION OF A 33-KILODALTON SECONDARY PROCESSING PRODUCT WHICH IS SHED PRIOR TO ERYTHROCYTE INVASION [J].
BLACKMAN, MJ ;
WHITTLE, H ;
HOLDER, AA .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 49 (01) :35-44
[4]   A SINGLE FRAGMENT OF A MALARIA MEROZOITE SURFACE PROTEIN REMAINS ON THE PARASITE DURING RED-CELL INVASION AND IS THE TARGET OF INVASION-INHIBITING ANTIBODIES [J].
BLACKMAN, MJ ;
HEIDRICH, HG ;
DONACHIE, S ;
MCBRIDE, JS ;
HOLDER, AA .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (01) :379-382
[5]   THE 3' PORTION OF THE GENE FOR A PLASMODIUM-YOELII MEROZOITE SURFACE-ANTIGEN ENCODES THE EPITOPE RECOGNIZED BY A PROTECTIVE MONOCLONAL-ANTIBODY [J].
BURNS, JM ;
DALY, TM ;
VAIDYA, AB ;
LONG, CA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (02) :602-606
[6]  
BURNS JM, 1989, J IMMUNOL, V143, P2670
[7]  
CASSANOVA JL, 1990, NUCLEIC ACIDS RES, V18, P4028
[8]   MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF THE GENE ENCODING THE MAJOR MEROZOITE SURFACE-ANTIGEN OF PLASMODIUM-CHABAUDI-CHABAUDI IP-PC1 [J].
DELEERSNIJDER, W ;
HENDRIX, D ;
BENDAHMAN, N ;
HANEGREEFS, J ;
BRIJS, L ;
HAMERSCASTERMAN, C ;
HAMERS, R .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 43 (02) :231-244
[9]   EGF-LIKE DOMAINS IN EXTRACELLULAR-MATRIX PROTEINS - LOCALIZED SIGNALS FOR GROWTH AND DIFFERENTIATION [J].
ENGEL, J .
FEBS LETTERS, 1989, 251 (1-2) :1-7
[10]   MITOCHONDRIAL-DNA OF THE HUMAN MALARIAL PARASITE PLASMODIUM-FALCIPARUM [J].
GARDNER, MJ ;
BATES, PA ;
LING, IT ;
MOORE, DJ ;
MCCREADY, S ;
GUNASEKERA, MBR ;
WILSON, RJM ;
WILLIAMSON, DH .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 31 (01) :11-17
123