EFFECT OF STARVATION AND DIABETES ON THE ACTIVITY OF THE EUKARYOTIC INITIATION-FACTOR EIF-2 IN RAT SKELETAL-MUSCLE

被引：36
|
作者：
JEFFREY, IW
KELLY, FJ
DUNCAN, R
HERSHEY, JWB
PAIN, VM
机构：
[1] UNIV SUSSEX, SCH BIOL SCI, BIOCHEM LAB, BRIGHTON BN1 9QG, E SUSSEX, ENGLAND
[2] UNIV CALIF DAVIS, DEPT BIOL CHEM, DAVIS, CA 95616 USA
来源：
BIOCHIMIE | 1990年 / 72卷 / 10期
关键词：
PROTEIN SYNTHESIS; INITIATION; STARVATION; INSULIN; SKELETAL MUSCLE; PROTEIN PHOSPHORYLATION;
D O I：
10.1016/0300-9084(90)90160-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ability of the initiation factor eIF-2 in skeletal muscle extracts to form ternary initiation complexes ([Met-tRNA(f).eIF-2.GDP]) is decreased by either starvation or diabetes. These conditions also impair the ability of muscle extracts to dissociate [eIF-2.GDP], suggesting inhibition of the guanine nucleotide exchange reaction essential for eIF-2 recycling. We could not, however, detect any change in the phosphorylation state of the alpha-subunit off eIF-2. This suggests that e-IF-2 activity may be regulated in this system by a mechanism not involving its phosphorylation.
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页码：751 / 757
页数：7
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