MODIFIED AMYLOID-BETA PROTEIN ENDING AT 42 OR 40 WITH DIFFERENT SOLUBILITY ACCUMULATES IN THE BRAIN OF ALZHEIMERS-DISEASE

被引：52
作者：
HARIGAYA, Y
SHOJI, M
KAWARABAYASHI, T
KANAI, M
NAKAMURA, T
IIZUKA, T
IGETA, Y
SAIDO, TC
SAHARA, N
MORI, H
HIRAI, S
机构：
[1] GUNMA UNIV,SCH MED,DEPT NEUROL,MAEBASHI,GUMMA 371,JAPAN
[2] TOKYO METROPOLITAN INST MED SCI,DEPT MOLEC BIOL,BUNKYO KU,TOKYO 113,JAPAN
[3] TOKYO INST PSYCHIAT,DEPT MOLEC BIOL,SETAGAYA KU,TOKYO 156,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 211卷 / 03期
关键词：
D O I：
10.1006/bbrc.1995.1912
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Serial extraction study of Alzheimer's disease (AD) and control brains revealed 4, 3.7 and 3 kD amyloid beta protein (A beta) species accumulated in AD brains. In the fractions extracted with TBS, 10% SDS and formic acid, considerable amounts of A beta species were recovered in SDS fractions besides TBS and formic acid tractions. Immunoblotting with several site-specific antibodies confirmed not only the presence of 4 kD A beta starting at the first amino acid of A beta but also 2 smaller A beta species with modification of their amino-termini in the highly resolutional Tris/Tricine gel system. A beta solubility using these solvents was associated with both modification of the amino-terminus and length of carboxyl-terminus of A beta. Especially, a large amount of modified A beta was found to be accumulated as forms with different solubility in AD brains. (C) 1995 Academic Press, Inc.
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页码：1015 / 1022
页数：8
相关论文
共 24 条
[1]   RELEASE OF EXCESS AMYLOID BETA-PROTEIN FROM A MUTANT AMYLOID BETA-PROTEIN PRECURSOR [J].
CAI, XD ;
GOLDE, TE ;
YOUNKIN, SG .
SCIENCE, 1993, 259 (5094) :514-516
[2]   MUTATION OF THE BETA-AMYLOID PRECURSOR PROTEIN IN FAMILIAL ALZHEIMERS-DISEASE INCREASES BETA-PROTEIN PRODUCTION [J].
CITRON, M ;
OLTERSDORF, T ;
HAASS, C ;
MCCONLOGUE, L ;
HUNG, AY ;
SEUBERT, P ;
VIGOPELFREY, C ;
LIEBERBURG, I ;
SELKOE, DJ .
NATURE, 1992, 360 (6405) :672-674
[3]   ALZHEIMERS-DISEASE - INITIAL REPORT OF THE PURIFICATION AND CHARACTERIZATION OF A NOVEL CEREBROVASCULAR AMYLOID PROTEIN [J].
GLENNER, GG ;
WONG, CW .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 120 (03) :885-890
[4]  
GOWING E, 1994, J BIOL CHEM, V269, P10987
[5]   AMYLOID BETA-PEPTIDE IS PRODUCED BY CULTURED-CELLS DURING NORMAL METABOLISM [J].
HAASS, C ;
SCHLOSSMACHER, MG ;
HUNG, AY ;
VIGOPELFREY, C ;
MELLON, A ;
OSTASZEWSKI, BL ;
LIEBERBURG, I ;
KOO, EH ;
SCHENK, D ;
TEPLOW, DB ;
SELKOE, DJ .
NATURE, 1992, 359 (6393) :322-325
[6]   VISUALIZATION OF A-BETA-42(43) AND A-BETA-40 IN SENILE PLAQUES WITH END-SPECIFIC A-BETA MONOCLONALS - EVIDENCE THAT AN INITIALLY DEPOSITED SPECIES IS A-BETA-42(43) [J].
IWATSUBO, T ;
ODAKA, A ;
SUZUKI, N ;
MIZUSAWA, H ;
NUKINA, N ;
IHARA, Y .
NEURON, 1994, 13 (01) :45-53
[7]   THE CARBOXY TERMINUS OF THE BETA-AMYLOID PROTEIN IS CRITICAL FOR THE SEEDING OF AMYLOID FORMATION - IMPLICATIONS FOR THE PATHOGENESIS OF ALZHEIMERS-DISEASE [J].
JARRETT, JT ;
BERGER, EP ;
LANSBURY, PT .
BIOCHEMISTRY, 1993, 32 (18) :4693-4697
[8]   DIAGNOSIS OF ALZHEIMERS-DISEASE [J].
KHACHATURIAN, ZS .
ARCHIVES OF NEUROLOGY, 1985, 42 (11) :1097-1104
[9]  
Kim K. S., 1988, NEUROSCI RES COMMUN, V2, P121
[10]  
KIM KS, 1990, NEUROSCI RES COMMUN, V7, P113
123