Improved Detection and Fragmentation of Disulphide-Linked Peptides

被引：1

作者：

Maes, Evelyne ^{[1
]}

Clerens, Stefan ^{[1
,2
]}

Dyer, Jolon M. ^{[1
,2
,3
,4
]}

Deb-Choudhury, Santanu ^{[1
]}

机构：

[1] AgResearch Ltd, Food Bio Based Prod, 1365 Springs Rd, Lincoln 7674, New Zealand

[2] Univ Canterbury, Biomol Interact Ctr, Christchurch 8140, New Zealand

[3] Massey Univ, Riddet Inst, Palmerston North 4442, New Zealand

[4] Lincoln Univ, Wine Food & Mol Biosci, Lincoln 7674, New Zealand

来源：

METHODS AND PROTOCOLS | 2018年 / 1卷 / 03期

关键词：

disulphide bonds; MALDI-TOF; TOF MS; aniline; proof-of-existence;

D O I：

10.3390/mps1030033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.

引用

页数：11

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