TARGETED EXPRESSION OF TETANUS TOXIN LIGHT-CHAIN IN DROSOPHILA SPECIFICALLY ELIMINATES SYNAPTIC TRANSMISSION AND CAUSES BEHAVIORAL DEFECTS

被引：685
作者：
SWEENEY, ST
BROADIE, K
KEANE, J
NIEMANN, H
OKANE, CJ
机构：
[1] UNIV CAMBRIDGE,DEPT ZOOL,CAMBRIDGE CB2 3EH,ENGLAND
[2] BUNDESFORSCH ANSTALT VIRUSKRANKHEITEN TIERE,D-72001 TUBINGEN,GERMANY
来源：
NEURON | 1995年 / 14卷 / 02期
基金：
英国惠康基金;
关键词：
D O I：
10.1016/0896-6273(95)90290-2
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Tetanus toxin cleaves the synaptic vesicle protein synaptobrevin, and the ensuing loss of neurotransmitter exocytosis has implicated synaptobrevin in this process. To further the study of synaptic function in a genetically tractable organism and to generate a tool to disable neuronal communication for behavioural studies, we have expressed a gene encoding tetanus toxin light chain in Drosophila. Toxin expression in embryonic neurons removes detectable synaptobrevin and eliminates evoked, but not spontaneous, synaptic vesicle release. No other developmental or morphological defects are detected. Correspondingly, only synaptobrevin (n-syb), but not the ubiquitously expressed syb protein, is cleaved by tetanus toxin in vitro. Targeted expression of toxin can produce specific behavioral defects; in one case, the olfactory escape response is reduced.
引用
收藏
页码：341 / 351
页数：11
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