OVEREXPRESSION OF ESCHERICHIA-COLI SINGLE-STRANDED-DNA BINDING-PROTEIN UNDER BACTERIOPHAGE-T-7 PROMOTER

被引：0

作者：

KISHORE, U ^{[1
]}

CHAUHAN, M ^{[1
]}

CHOUDHURY, NR ^{[1
]}

KHATRI, GS ^{[1
]}

机构：

[1] JAWAHARLAL NEHRU UNIV, CTR BIOTECHNOL, GENET ENGN UNIT, NEW DELHI 110067, INDIA

来源：

INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS | 1994年 / 31卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant vector for overproduction of the E. coli single stranded DNA binding protein (E. coli SSBP) has been constructed. An E. coli strain carrying this plasmid produces up to 15.0 mg pure SSBP per litre of bacterial culture in a laboratory shake flask. Electron microscopy of the single stranded DNA complexed with SSBP shows characteristic ''beaded string''-like appearance. Strong clustering of protein molecules on ssDNA is indicative of a highly cooperative binding.

引用

页码：249 / 253

页数：5

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