CHARACTERIZATION OF HUMAN TUMOR-CELL LINES USING ANTIBODIES TO INTERMEDIATE FILAMENTS

被引：2
|
作者：
MOORTHI, C [1 ]
WILLERS, I [1 ]
RESSLER, B [1 ]
GOEDDE, HW [1 ]
机构：
[1] UNIV HAMBURG,INST HUMAN GENET,BUTENFELD 32,D-22529 HAMBURG,GERMANY
来源：
PATHOBIOLOGY | 1993年 / 61卷 / 3-4期
关键词：
TUMOR CELL LINES; INTERMEDIATE FILAMENTS; IMMUNOCYTOCHEMICAL METHODS;
D O I：
10.1159/000163793
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The human hepatoma cell line G2 (Hep G2) has been compared to lung carcinoma, sarcoma and skin fibroblasts for the expression of intermediate filaments, i.e. vimentin and cytokeratin. The immunofluorescence study revealed that, in contradiction to Szecheng et al. (1987), cytokeratin and vimentin are absent in Hep G2. Human skin fibroblasts and sarcoma cells expressed vimentin as expected for their mesenchymal origin, but a positive reaction to vimentin could also be shown in lung carcinoma cells. However, the vimentin filament structure of both these tumour cell lines was different in comparison with skin fibroblasts. Therefore determining the exact tissue origin of tumour cell lines by means of intermediate filament characterization remains doubtful.
引用
收藏
页码：197 / 199
页数：3
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