COMBINED BIOCHEMICAL AND IMMUNOCYTOCHEMICAL ANALYSES OF POSTMORTEM PROTEIN DEIMINATION IN THE RAT SPINAL-CORD

被引：41
作者：
ASAGA, H
SENSHU, T
机构：
[1] Department of Cell Chemistry, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, 173
来源：
CELL BIOLOGY INTERNATIONAL | 1993年 / 17卷 / 05期
关键词：
D O I：
10.1006/cbir.1993.1094
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Immunocytochemical staining of citrulline-containing proteins, i.e. products of endogenous peptidylarginine deiminase (EC 3.5.3.15) reaction, has been performed by chemical modification of citrulline residues in situ, followed by probing with IgG specific to the modified residues. Rat spinal cords that had been preincubated in vitro to accelerate the enzyme reaction were used as samples. Not only astrocytes but also the cytoplasm of some large neurons were stained. Usefulness of the method for studying nervous tissue damage was suggested. © 1993 Academic Press. All rights reserved.
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页码：525 / 532
页数：8
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