ON HETEROGENEITY OF HEMOGLOBIN FROM NORMAL AND GLUCOSE-6-PHOSPHATE DEHYDROGENASE-DEFICIENT INDIVIDUALS

Quantitative measurements of the different hemoglobin fractions obtained by CM-cellulose and Amberlite CG-50 chromatography and cellogel electrophoresis from normal and glucose-6-phosphate dehydrogenase-deficient adults are presented. The mean values, obtained by the three procedures, for the Hb-A3 fraction of glucose-6-phosphate dehydrogenase-deucient red blood cells was lower than in normal red blood cells. (The difference was statistically significant in separations on CM-cellulose and cellogel electrophoresis). The mean values for the Hb-A1 fraction, obtained by cellogel electrophoresis were significantly higher than m normal red blood cells. No significant difference in the amount of the Hb-A2 fraction of the two kinds of red blood cells was observed. The significance of these results is discussed. © 1969.