Genome Sequence of a Tomato-Infecting Tomato Mosaic Virus Isolate from Zimbabwe

被引:0
作者
Karavina, Charles [1 ,2 ]
Ibaba, Jacques D. [3 ]
Gubba, Augustine [1 ]
机构
[1] Univ KwaZulu Natal, Dept Plant Pathol, Pietermaritzburg, Kwazulu Natal, South Africa
[2] Bindura Univ Sci Educ, Dept Crop Sci, Bindura, Zimbabwe
[3] Univ Pretoria, Ctr Microbial Ecol & Genom, Genom Res Inst, Pretoria, South Africa
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D O I
10.1128/genomeA.01457-17
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摘要
A tomato-infecting tomato mosaic virus (ToMV) isolate was detected in Zimbabwe using lateral flow kits and double-antibody sandwich enzyme-linked immunosorbent assay. Next-generation sequencing and de novo assembly were subsequently performed to determine its genome sequence. The ToMV genome of the Zimbabwe isolate is the second to be reported in Africa.
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共 8 条
[1]   SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing [J].
Bankevich, Anton ;
Nurk, Sergey ;
Antipov, Dmitry ;
Gurevich, Alexey A. ;
Dvorkin, Mikhail ;
Kulikov, Alexander S. ;
Lesin, Valery M. ;
Nikolenko, Sergey I. ;
Son Pham ;
Prjibelski, Andrey D. ;
Pyshkin, Alexey V. ;
Sirotkin, Alexander V. ;
Vyahhi, Nikolay ;
Tesler, Glenn ;
Alekseyev, Max A. ;
Pevzner, Pavel A. .
JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) :455-477
[2]   Trimmomatic: a flexible trimmer for Illumina sequence data [J].
Bolger, Anthony M. ;
Lohse, Marc ;
Usadel, Bjoern .
BIOINFORMATICS, 2014, 30 (15) :2114-2120
[3]  
Dobson H, 2002, INTEGRATED VEGETABLE
[4]   Detection of Tobacco mosaic virus and Tomato mosaic virus in pepper and tomato by multiplex RT-PCR [J].
Kumar, S. ;
Shankar, A. C. Udaya ;
Nayaka, S. C. ;
Lund, O. S. ;
Prakash, H. S. .
LETTERS IN APPLIED MICROBIOLOGY, 2011, 53 (03) :359-363
[5]  
Duarte Lígia Maria Lembo, 2007, Summa phytopathol., V33, P409, DOI 10.1590/S0100-54052007000400016
[6]   RDP4: Detection and analysis of recombination patterns in virus genomes [J].
Martin, Darren P. ;
Murrell, Ben ;
Golden, Michael ;
Khoosal, Arjun ;
Muhire, Brejnev .
VIRUS EVOLUTION, 2015, 1 (01)
[7]   The double-resistance-breaking Tomato mosaic virus strain ToMV1-2 contains two independent single resistance-breaking domains [J].
Strasser, M. ;
Pfitzner, A. J. P. .
ARCHIVES OF VIROLOGY, 2007, 152 (05) :903-914
[8]  
Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/mst197, 10.1093/molbev/msr121]