KINETIC-ANALYSIS OF TIME-RESOLVED INFRARED DIFFERENCE SPECTRA OF THE L-INTERMEDIATES AND M-INTERMEDIATES OF BACTERIORHODOPSIN

被引:71
作者
CHEN, WG [1 ]
BRAIMAN, MS [1 ]
机构
[1] UNIV VIRGINIA,HLTH SCI CTR,DEPT BIOCHEM,CHARLOTTESVILLE,VA 22908
关键词
D O I
10.1111/j.1751-1097.1991.tb02110.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Infrared difference spectra with 4 cm-1 spectral resolution and 10-mu-s temporal resolution, obtained previously with a stroboscopic Fourier-transform difference technique (Braiman et al., 1991, Proc. Natl. Acad. Sci. USA 88, 2388), were analyzed by means of a global exponential fitting procedure based on singular value decomposition. Using a simple linear kinetic model K --> L --> M for the bacteriorhodopsin (bR) photocycle in the time range 10-1000-mu-s at 16.5-degrees-C, it was possible to generate bR --> L and bR --> M difference spectra with signal/noise ratios comparable to those obtainable with low temperature difference spectroscopy. The resulting time-resolved bR --> L and bR --> M difference spectra are both very similar to the corresponding static FTIR difference spectra obtained at 175 K and 250 K, respectively. In the bR --> L spectrum, however, there are interesting differences that may indicate a greater degree of deprotonation of Asp-96 when L is formed at physiological temperatures than when it is observed in a low-temperature steady state.
引用
收藏
页码:905 / 910
页数:6
相关论文
共 14 条
[1]   THE ROLE OF BACK-REACTIONS AND PROTON UPTAKE DURING THE N-]O TRANSITION IN BACTERIORHODOPSINS PHOTOCYCLE - A KINETIC RESONANCE RAMAN-STUDY [J].
AMES, JB ;
MATHIES, RA .
BIOCHEMISTRY, 1990, 29 (31) :7181-7190
[2]  
BOUSCHE O, 1991, J BIOL CHEM, V266, P11063
[3]   MILLISECOND FOURIER-TRANSFORM INFRARED DIFFERENCE SPECTRA OF BACTERIORHODOPSINS M412 PHOTOPRODUCT [J].
BRAIMAN, MS ;
AHL, PL ;
ROTHSCHILD, KJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (15) :5221-5225
[4]   VIBRATIONAL SPECTROSCOPY OF BACTERIORHODOPSIN MUTANTS - LIGHT-DRIVEN PROTON TRANSPORT INVOLVES PROTONATION CHANGES OF ASPARTIC-ACID RESIDUE-85, RESIDUE-96, AND RESIDUE-212 [J].
BRAIMAN, MS ;
MOGI, T ;
MARTI, T ;
STERN, LJ ;
KHORANA, HG ;
ROTHSCHILD, KJ .
BIOCHEMISTRY, 1988, 27 (23) :8516-8520
[5]   PROTEIN DYNAMICS IN THE BACTERIORHODOPSIN PHOTOCYCLE - SUBMILLISECOND FOURIER-TRANSFORM INFRARED-SPECTRA OF THE L-PHOTOINTERMEDIATES, M-PHOTOINTERMEDIATES, AND N-PHOTOINTERMEDIATES [J].
BRAIMAN, MS ;
BOUSCHE, O ;
ROTHSCHILD, KJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2388-2392
[6]   FTIR DIFFERENCE STUDIES ON APOPROTEINS - PROTONATION STATES OF ASPARTIC AND GLUTAMIC-ACID RESIDUES DURING THE PHOTOCYCLE OF BACTERIORHODOPSIN [J].
EISENSTEIN, L ;
LIN, SL ;
DOLLINGER, G ;
ODASHIMA, K ;
TERMINI, J ;
KONNO, K ;
DING, WD ;
NAKANISHI, K .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (22) :6860-6862
[7]   LIGHT-DRIVEN PROTONATION CHANGES OF INTERNAL ASPARTIC ACIDS OF BACTERIORHODOPSIN - AN INVESTIGATION BY STATIC AND TIME-RESOLVED INFRARED DIFFERENCE SPECTROSCOPY USING [4-C-13] ASPARTIC ACID LABELED PURPLE MEMBRANE [J].
ENGELHARD, M ;
GERWERT, K ;
HESS, B ;
KREUTZ, W ;
SIEBERT, F .
BIOCHEMISTRY, 1985, 24 (02) :400-407
[8]   ROLE OF ASPARTATE-96 IN PROTON TRANSLOCATION BY BACTERIORHODOPSIN [J].
GERWERT, K ;
HESS, B ;
SOPPA, J ;
OESTERHELT, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (13) :4943-4947
[9]   PHOTOCYCLES OF BACTERIORHODOPSIN IN LIGHT-ADAPTED AND DARK-ADAPTED PURPLE MEMBRANE STUDIED BY TIME-RESOLVED ABSORPTION-SPECTROSCOPY [J].
HOFRICHTER, J ;
HENRY, ER ;
LOZIER, RH .
BIOPHYSICAL JOURNAL, 1989, 56 (04) :693-706
[10]  
HOFRICHTER J, 1985, BIOCHEMISTRY-US, V24, P2667, DOI 10.1021/bi00332a012