PCNA IMMUNOSTAINING COMBINED WITH AGNOR STAINING IN ESOPHAGEAL SQUAMOUS-CELL CARCINOMA TO IDENTIFY PATIENTS WITH A POOR-PROGNOSIS

被引：14

作者：

MORISAKI, Y

SHIMA, S

YOSHIZUMI, Y

SUGIURA, Y

TANAKA, S

TAMAI, S

机构：

[1] Department of Surgery II, National Defense Medical College, Saitama, 359, 3-2 Namiki, Tokorozawa

[2] Department of Laboratory Medicine, National Defense Medical College, Saitama, 359, 3-2 Namiki, Tokorozawa

来源：

SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY | 1995年 / 25卷 / 05期

关键词：

PCNA; AGNOR; PROGNOSIS; ESOPHAGEAL CANCER;

D O I：

10.1007/BF00311814

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Immunostaining of the proliferating cell nuclear antigen (PCNA) provides important information about cell kinetics and is easily performed on routinely obtained formalin-fixed, paraffin-embedded materials. We report herein the results of a retrospective study on PCNA staining in esophageal cancer undertaken to determine its significance. As this study indicated that immunoreactivity was preserved in specimens fixed within 24 h, only 31 specimens from surgical patients were available for this investigation. The mean PCNA index of the patients without invasion to the adventitia (35.7 +/- 17.9) was significantly lower than that of those with invasion to the adventitia or neighboring structures (49.7 +/- 14.5), while the PCNA index did not correlate with other clinicopathologic parameters such as histologic type, lymph node metastases, or prognosis. However, when an analysis of PCNA staining was combined with an analysis of argyrophilic nucleolar organizer region (AgNOR) staining, a correlation with prognosis was found. In fact, seven patients with a high PCNA index (greater-than-or-equal-to 44) and AgNOR count (greater-than-or-equal-to 6) had a significantly poorer prognosis than the remaining 22 (P = 0.0014), and six of these seven patients died within 2 years. These results indicate that this combined evaluation may be useful for the identification of patients with a poor prognosis among those undergoing surgery for esophageal squamous cell carcinoma.

引用

页码：389 / 395

页数：7

共 27 条

[1]

Penneys N.S., Bogaet M., Serfling U., Sisto M., PCNA expression in cutaneous keratinous neoplasms and verruca vulgaris, Am J Pathol, 141, pp. 139-142, (1992)

[2]

Wilson G.D., McNally N.J., Dishe S., Saunders M.I., Des Rocher C., Lewis A.A., Bennet M.H., Measurement of cell kinetics in human tumors in vivo using bromodeoxyuridine incorporation and flow cytometry, Br J Cancer, 58, pp. 423-431, (1988)

[3]

Yoshida K., Morinaga S., Shimosato Y., Hayata Y., A cell kinetic study of pulmonary adenocarcinoma by an immunoperoxidase procedure after bromodeoxyuridine labeling, Cancer, 64, pp. 2284-2291, (1989)

[4]

Alama A., Massimo C., Reppetto L., Conte P.F., Serrano J., Nicolin A., Barbieri F., Ardizzoni A., Thymidine labelling index as prognostic factor in resected non-small cell lung cancer, Eur J Cancer, 26, pp. 622-625, (1990)

[5]

Sahin A.A., Ro J., Ro J.Y., Blick M.B., El-Naggar K., Ordonez N.G., Fritsche H.A., Smith T.L., Hortobagyi G.N., Ayala A.G., Ki67 immunostaining in node-negative stage I/II breast carcinoma, Cancer, 68, pp. 549-557, (1991)

[6]

Tateishi M., Ishida T., Mitsudomi T., Sugimachi K., DNA polymerase-α as a putative early relapse marker in non-small cell lung cancer, Cancer, 68, pp. 925-929, (1991)

[7]

Robins B.A., Vega D., Ogata K., Tan E.M., Nakamura R.M., Immunohistochemical detection of proliferating cell nuclear antigen in solid human malignancies, Arch Pathol Lab Med, 111, pp. 841-845, (1987)

[8]

Crocker J., Macartney J.C., Smith L., Correlation between DNA flow cytometric and nucleolar organizer region data in non-Hodgkin's lymphomas, J Pathol, 154, pp. 151-156, (1988)

[9]

Atkin N.B., Kay R., Prognostic significance of model DNA value and other factors in malignant tumors, based on 1,465 cases, Br J Cancer, 40, pp. 210-221, (1979)

[10]

Matsuura H., Sugimachi K., Ueo H., Kuwano H., Koga Y., Okamura T., Malignant potentiality of squamous cell carcinoma of the esophagus predictable by DNA anaysis, Cancer, 57, pp. 1810-1814, (1986)

← 1 2 3 →