INTRAVENOUS DOSE-LETHALITY STUDY OF AMERICAN PIT VIPER VENOMS IN MICE USING STANDARDIZED METHODS

被引：11

作者：

CONSROE, P

GERRISH, K

EGEN, N

RUSSELL, FE

机构：

[1] Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona, Tucson, Arizona

来源：

JOURNAL OF WILDERNESS MEDICINE | 1992年 / 3卷 / 02期

关键词：

AMERICAN PIT VIPERS; VENOMS; LD50; MICE; SNAKES; ANTIVENIN;

D O I：

10.1580/0953-9859-3.2.162

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

An intravenous dose-lethality study of 14 clinically important North and South American pit viper venoms was carried out in mice using standardized, rigorous protocols developed to meet international (WHO) guidelines and US government (FDA) regulations for the development of new treatment products. The study was undertaken in order to establish baseline LD50 values for the ongoing testing of a new crotalid antivenin F(AB) prepared from sheep immunoglobulin (IgG). The venoms (lyophilized and pooled from juvenile and adult snakes of both sexes) were taken from 10 rattlesnake (nine Crotalus and one Sistrurus) species, two Agkistrodon species, and two Bothrops species. The dose-response data were subjected to probit analysis. Median lethal dose (LD50) values and their 95% confidence limits (CL) were calculated and statistical comparisons between the median potencies were made. The intravenous LD50s (and 95% CL) ranged from a low of 0.13 (0.10-0.15) mg kg-1 for Crotalus durissus terrificus, to a high of 6.32 (5.76-6.94) mg kg-1 for Crotalus horridus horridus. Significant positive rank-order correlations were also found for comparisons between the present LD50s and published LD50s for intravenous, intraperitoneal, and subcutaneous administration.

引用

页码：162 / 167

页数：6

共 20 条

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