TYROSYL PHOSPHORYLATION AND ACTIVATION OF MAP KINASES BY P56LCK

被引:137
作者
ETTEHADIEH, E
SANGHERA, JS
PELECH, SL
HESSBIENZ, D
WATTS, J
SHASTRI, N
AEBERSOLD, R
机构
[1] UNIV BRITISH COLUMBIA, BIOMED RES CTR, VANCOUVER V6T 1Z3, BC, CANADA
[2] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA
[3] UNIV BRITISH COLUMBIA, DEPT BIOCHEM, VANCOUVER V6T 1Z3, BC, CANADA
[4] UNIV BRITISH COLUMBIA, DEPT MED, VANCOUVER V6T 1Z3, BC, CANADA
关键词
D O I
10.1126/science.1311128
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
T cell signaling via the CD4 surface antigen is mediated by the associated tyrosyl protein kinase p56lck. The 42-kilodalton mitogen-activated protein (MAP) kinase (p42mapk) was tyrosyl-phosphorylated and activated after treatment of the murine T lymphoma cell line 171CD4+, which expresses CD4, with antibody to CD3. Treatment of the CD4-deficient cell line 171 with the same antibody did not result in phosphorylation or activation of p42mapk. Purified p56lck both tyrosyl-phosphorylated and stimulated the seryl-threonyl phosphotransferase activity of purified p44mpk, a MAP kinase isoform from sea star oocytes. A synthetic peptide modeled after the putative regulatory phosphorylation site in murine p42mapk (Tyr185) was phosphorylated by p56lck with a similar V(max), but a fivefold lower Michaelis constant (K(m)) than a peptide containing the Tyr394 autophosphorylation site from p56lck. MAP kinases may participate in protein kinase cascades that link Src family protein-tyrosyl kinases to seryl-threonyl kinases such as those encoded by rsk and raf, which are putative substrates of MAP kinases.
引用
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页码:853 / 855
页数:3
相关论文
共 48 条
[1]  
AHN NG, 1991, J BIOL CHEM, V256, P11487
[2]   RAF-1 IS A POTENTIAL SUBSTRATE FOR MITOGEN-ACTIVATED PROTEIN-KINASE INVIVO [J].
ANDERSON, NG ;
PING, LI ;
MARSDEN, LA ;
WILLIAMS, N ;
ROBERTS, TM ;
STURGILL, TW .
BIOCHEMICAL JOURNAL, 1991, 277 :573-576
[3]   REQUIREMENT FOR INTEGRATION OF SIGNALS FROM 2 DISTINCT PHOSPHORYLATION PATHWAYS FOR ACTIVATION OF MAP KINASE [J].
ANDERSON, NG ;
MALLER, JL ;
TONKS, NK ;
STURGILL, TW .
NATURE, 1990, 343 (6259) :651-653
[4]  
ANDERSON P, 1988, J IMMUNOL, V140, P1732
[5]   PURIFICATION AND PROPERTIES OF EXTRACELLULAR SIGNAL-REGULATED KINASE-1, AN INSULIN-STIMULATED MICROTUBULE-ASSOCIATED PROTEIN-2 KINASE [J].
BOULTON, TG ;
GREGORY, JS ;
COBB, MH .
BIOCHEMISTRY, 1991, 30 (01) :278-286
[6]   IDENTIFICATION OF MULTIPLE EXTRACELLULAR SIGNAL-REGULATED KINASES (ERKS) WITH ANTIPEPTIDE ANTIBODIES [J].
BOULTON, TG ;
COBB, MH .
CELL REGULATION, 1991, 2 (05) :357-371
[7]   AN INSULIN-STIMULATED PROTEIN-KINASE SIMILAR TO YEAST KINASES INVOLVED IN CELL-CYCLE CONTROL [J].
BOULTON, TG ;
YANCOPOULOS, GD ;
GREGORY, JS ;
SLAUGHTER, C ;
MOOMAW, C ;
HSU, J ;
COBB, MH .
SCIENCE, 1990, 249 (4964) :64-67
[8]   ERKS - A FAMILY OF PROTEIN-SERINE THREONINE KINASES THAT ARE ACTIVATED AND TYROSINE PHOSPHORYLATED IN RESPONSE TO INSULIN AND NGF [J].
BOULTON, TG ;
NYE, SH ;
ROBBINS, DJ ;
IP, NY ;
RADZIEJEWSKA, E ;
MORGENBESSER, SD ;
DEPINHO, RA ;
PANAYOTATOS, N ;
COBB, MH ;
YANCOPOULOS, GD .
CELL, 1991, 65 (04) :663-675
[9]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[10]  
CHAREST D, IN PRESS