RAPID, SENSITIVE DISK ASSAY FOR THE DETERMINATION OF GLYCOPROTEIN GLYCOSYLTRANSFERASES

被引：44

作者：

BAXTER, A

DURHAM, JP

机构：

[1] Department of Clinical Oncology, University of Glasgow, Glasgow, G12 9LY Scotland

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0003-2697(79)90711-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive disk assay of glycoprotein glycosyltransferase activity has been developed. The labeled acceptor protein is isolated on a cellulose paper disk by fixing and washing with a protein precipitant solution. The effect of protein and detergent concentration on the capacity of the disk for the acceptor protein has been determined. The method is compared with others in current use and the advantages of the disk method are discussed. © 1979.

引用

页码：95 / 101

页数：7

共 11 条

[1]

BOLLUM FJ, 1959, J BIOL CHEM, V234, P2733

[2] A SIMPLE COLORIMETRIC METHOD FOR DETERMINATION OF PROTEIN [J].