HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR BASIC AMINE DRUGS IN HUMAN PLASMA WITH A SILICA-GEL COLUMN AND AN AQUEOUS MOBILE PHASE .4. ALBUTEROL

被引：7

作者：

WU, YQ ^{[1
]}

SHI, R ^{[1
]}

WILLIAMS, RL ^{[1
]}

LIN, ET ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,SCH PHARM,DEPT PHARM,SAN FRANCISCO,CA 94143

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 02期

关键词：

D O I：

10.1080/01483919108049612

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high performance liquid chromatographic (HPLC) assay is presented for the determination of albuterol in human plasma. Sample analysis is performed with a silica gel column and an aqueous mobile phase. Plasma samples are extracted with chloroform containing di(2-ethylhexyl) phosphate. Samples are back extracted into a hydrochloric acid solution. A fluorescence detector is used for this assay. The detection limit of the assay is 0.2 ng/ml for 0.5 ml plasma samples. This method has been used in a bioavailability study of albuterol sulfate tablets (4.8 mg) given to healthy volunteers.

引用

页码：253 / 264

页数：12

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