PRELIMINARY EXPERIMENTS IN THE SEPARATION OF GLOBIN CHAINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：45
作者：
SHELTON, JB
SHELTON, JR
SCHROEDER, WA
机构：
[1] Division of Chemistry and Chemical Engineering (Contribution 6075), California Institute of Technology, Pasadena, CA
来源：
HEMOGLOBIN | 1979年 / 3卷 / 05期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.3109/03630267908997540
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The power of high performance liquid chromatography (HPLC) for the separation of complex mixtures of peptides from proteins is becoming increasingly evident (1-4 and references in 3). However, the application of HPLC to the separation of proteins is limited. Mönch and Dehnen (5) separated artificial mixtures of proteins (5,400 to 450,000 daltons) and Rubenstein et al. used HPLC to purify interferon (6). In preliminary experiments, we have had some success in the separation of human globin chains by HPLC. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码：353 / 358
页数：6
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