FUNDAMENTALS OF QUANTITATIVE AUTORADIOGRAPHY BY COMPUTER DENSITOMETRY FOR IN-SITU HYBRIDIZATION, WITH EMPHASIS ON P-33

被引：54

作者：

BASKIN, DG

STAHL, WL

机构：

[1] VET AFFAIRS MED CTR,NEUROCHEM LAB,SEATTLE,WA

[2] UNIV WASHINGTON,SCH MED,DEPT MED,SEATTLE,WA 98195

[3] UNIV WASHINGTON,SCH MED,DEPT BIOL STRUCT,SEATTLE,WA 98195

[4] UNIV WASHINGTON,SCH MED,DEPT PHYSIOL & BIOPHYS,SEATTLE,WA 98195

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1993年 / 41卷 / 12期

关键词：

IMAGE ANALYSIS; CALIBRATION; STANDARDIZATION;

D O I：

10.1177/41.12.8245425

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The goal of quantitative autoradiography (QAR) in in situ hybridization (ISH) is to determine the amount of radioactive oligonucleotide or riboprobe present in the corresponding area of the tissue slice that produced an autoradiographic image. This article discusses (a) some of the considerations related to selection and use of computer image analysis systems for accomplishing this objective, (b) development of C-14 plastic autoradiographic standards for ISH QAR with P-33, (c), using QAR to develop T-m curves for ISH probes, and (d) measurement of resolution with video imaging systems for QAR.

引用

页码：1767 / 1776

页数：10

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