ISOLATION AND IDENTIFICATION OF PRE-VITAMIN D3 FROM THE SKIN OF RATS EXPOSED TO ULTRAVIOLET-IRRADIATION

被引：89

作者：

HOLICK, MF ^{[1
]}

RICHTAND, NM ^{[1
]}

MCNEILL, SC ^{[1
]}

HOLICK, SA ^{[1
]}

FROMMER, JE ^{[1
]}

HENLEY, JW ^{[1
]}

POTTS, JT ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED, DEPT MED, BOSTON, MA 02120 USA

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 06期

关键词：

D O I：

10.1021/bi00573a011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The process of the photolytic activation of vitamin D precursor(s) in the skin has been elucidated by a detailed analysis of the products formed after ultraviolet light exposure. The photolytic product isolated from the skin of rats exposed to ultraviolet irradiation was identified as previtamin D3 by several criteria including its (a) characteristic ultraviolet absorption spectrum, (b) mass spectrum, and (c) thermal isomerization to vitamin D3, which itself was identified also by mass spectroscopy. Vitamin D3 per se was not formed by ultraviolet irradiation—vitamin D3 arises exclusively from the thermal conversion of previtamin D3. Detectable amounts of lumisterol3 or tachysterol3 were not seen. © 1979, American Chemical Society. All rights reserved.

引用

页码：1003 / 1008

页数：6

共 28 条

[1] The distillation of vitamin D
Askew, FA
Bourdillon, RB
Bruce, HM
Jenkins, RGC
Webster, TA
[J]. PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-CONTAINING PAPERS OF A BIOLOGICAL CHARACTER, 1930, 107 (748) : 76 - 90
[2] BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[3] SYNTHESIS OF 25-HYDROXYCHOLECALCIFEROL . A BIOLOGICALLY ACTIVE METABOLITE OF VITAMIN D3
BLUNT, JW
DELUCA, HF
[J]. BIOCHEMISTRY, 1969, 8 (02) : 671 - &
[4] METABOLISM AND MECHANISM OF ACTION OF VITAMIN-D1
DELUCA, HF
SCHNOES, HK
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1976, 45 : 631 - 666
[5] ELLINGBOE J, 1970, J LIPID RES, V11, P266
[6] VITAMIN-D3 FROM RAT SKINS IRRADIATED INVITRO WITH ULTRAVIOLET-LIGHT
ESVELT, RP
SCHNOES, HK
DELUCA, HF
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1978, 188 (02) : 282 - 286
[7] A study of rats on a normal diet irradiated daily by the mercury vapour quartz lamp or kept in darkness.
Goldblatt, H
Soames, KM
[J]. BIOCHEMICAL JOURNAL, 1923, 17 (02) : 294 - 297
[8] Studies on the fat-soluble growth-promoting factor. (I) Storage. (II) Synthesis.
Goldblatt, H
Soames, KM
[J]. BIOCHEMICAL JOURNAL, 1923, 17 (4-5) : 446 - 453
[9] BASIC AND CLINICAL CONCEPTS RELATED TO VITAMIN-D METABOLISM AND ACTION .1.
HAUSSLER, MR
MCCAIN, TA
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1977, 297 (18) : 974 - 983
[10] HAVINGA E, 1955, RECL TRAV CHIM PAY B, V74, P1230

← 1 2 3 →