ISOLATION OF SYNAPTOSOMAL FRACTIONS FROM RABBIT RETINA

被引：39

作者：

REDBURN, DA ^{[1
]}

THOMAS, TN ^{[1
]}

机构：

[1] MED UNIV S CAROLINA,DEPT BIOCHEM & PSYCHIAT,CHARLESTON,SC 29403

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1979年 / 1卷 / 03期

关键词：

D O I：

10.1016/0165-0270(79)90034-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Homogenization of rabbit retina in isotonic sucrose and differential rate centrifugation yielded two morphologically distinct synaptosomal fractions. One fraction was enriched in photoreceptor cell synaptosomes; the second fraction contained small synaptosomes derived from conventional sized synapses most abundant in the inner plexiform layer. Attempts to further purify these fractions using a variety of density gradients proved unsuccessful due to poor viability of photoreceptor cell synaptosomes. The synaptosomes prepared by our method are functionally stable as they demonstrate high affinity uptake for putative retinal neurotransmitters, neurotransmitter-sensitive adenylate cyclase activity, and calcium-dependent, potassium-stimulated release of [14C]GABA and [3H]dopamine. © 1979.

引用

页码：235 / 242

页数：8

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