CANATOXIN INDUCES ACTIVATION ON MICE PERITONEAL-MACROPHAGES

被引：0
作者：
GHAZALEH, FA [1 ]
ARAUJO, CF [1 ]
BARJAFIDALGO, C [1 ]
CARLINI, CR [1 ]
机构：
[1] UNIV ESTADO RIO DE JANEIRO,INST BIOL,DEPT FARMACOL,BR-20551-000 RIO JANEIRO,BRAZIL
来源：
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 1992年 / 25卷 / 10期
关键词：
MACROPHAGES; CANATOXIN; PHAGOCYTOSIS; N-ACETYL-BETA-D-GLUCOSAMINIDASE;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Canatoxin (CNTX), the toxic protein from Canavalia ensiformis seeds, injected into the peritoneal cavities of mice (10 mug/cavity) induced a significant neutrophil migration (10.5 +/- 0.5 x 10(6) cells/cavity) after 4 h. A later migratory effect (48 h) on mononuclear cells, predominantly macrophages, was also observed (controls: 7 +/- 0.9; CNTX: 17 +/- 2.0 x 10(6) cells/cavity). These CNTX-elicited macrophages, when compared to resident cells (R) or cells elicited by thioglycollate (TG), had an increased content of the lysosomal enzyme N-acetyl-beta-D-glucosaminidase (R: 4.5 +/- 0.5; TG: 7.2 +/- 1.0; CNTX: 20.2 +/- 3.0 mU/10(6) cells) and a greater (greater-than-or-equal-to 100%) phagocytic activity. The data suggest that CNTX-stimulated macrophages presented some characteristics of activated cells.
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收藏
页码：1033 / 1035
页数：3
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