EXPRESSION OF CYTOSKELETAL AND MATRIX GENES FOLLOWING EXPOSURE TO IONIZING-RADIATION - DOSE-RATE EFFECTS AND PROTEIN-SYNTHESIS REQUIREMENTS

被引：15

作者：

WOLOSCHAK, GE ^{[1
]}

FELCHER, P ^{[1
]}

CHANGLIU, CM ^{[1
]}

机构：

[1] LOYOLA UNIV, MED CTR, DEPT PATHOL, MAYWOOD, IL 60153 USA

来源：

CANCER LETTERS | 1995年 / 92卷 / 02期

关键词：

RADIATION DOSE-RATE EFFECTS; IONIZING RADIATION; DNA-DAMAGING AGENTS; STRESS RESPONSES; DAMAGE INDUCIBLE GENES; PROTEIN SYNTHESIS;

D O I：

10.1016/0304-3835(95)03767-Q

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Experiments were designed to examine the effects of radiation dose-rate and cycloheximide on expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results here demonstrated little effect of dose-rate for fission-spectrum neutrons when comparing expression of alpha-tubulin or fibronectin genes. Effects of cycloheximide, however, revealed several findings: (a) Cycloheximide repressed accumulation of alpha-tubulin following exposure to high dose-rate neutrons or gamma-rays. (b) Cycloheximide did not affect accumulation of mRNA for actin genes. (c) Cycloheximide abrogated the moderate induction of fibronectin-mRNA which occurred following exposure to gamma-rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of alpha-tubulin and fibronectin mRNA accumulation following exposure to ionizing radiation.

引用

页码：135 / 141

页数：7

共 24 条

[1]

Woloschak, Liu, Jones, Jones, Modulation of gene expression in Syrian hamster embryo cells following ionizing radiation, Cancer Res., 50, pp. 339-344, (1990)

[2]

Woloschak, Shearin-Jones, Chang-Liu, Effects of ionizing radiation on expression of genes encoding cytoskeletal elements: kinetics and dose effects, Mol. Carcinogenesis, 3, pp. 374-378, (1990)

[3]

Woloschak, Chang-Liu, Expression of cytoskeletal elements in proliferating cells following radiation exposure, Int. J. Radiat. Biol., 59, pp. 1173-1183, (1991)

[4]

Hollander, Fornace, Induction of fos RNA by DNA-damaging agents, Cancer Res., 49, pp. 1687-1692, (1989)

[5]

Anderson, Woloschak, Cellular proto-oncogene expression following exposure of mice to γ rays, Radiat. Res., 130, pp. 340-344, (1992)

[6]

Anscher, Crocker, Jirtle, Transforming growth factor-β-1 expression in irradiated liver, Radiat. Res., 122, pp. 77-85, (1992)

[7]

Woloschak, Liu, Shearin-Jones, Regulation of protein kinase C by ionizing radiation, Cancer Res., 50, pp. 3963-3967, (1990)

[8]

Fornace, Alamo, Hollander, DNA damage-inducible transcripts in mammalian cells, Proc. Natl. Acad. Sci. USA, 85, pp. 8800-8804, (1988)

[9]

Boothman, Pardee, Inhibition of radiation-induced neoplastic transformation by beta lapachone, Proc. Natl. Acad. Sci. USA, 86, pp. 4963-4967, (1989)

[10]

Fornace, Alamo, Hollander, Lamoreau, Ubiquitin RNA is a major stress-induced transcript in mammalian cells, Nucleic Acids Res., 17, pp. 1215-1230, (1989)

← 1 2 3 →