Peroxidase-catalyzed phenol removal, in which phenol is polymerized to form insoluble precipitates, was investigated in order to reduce the amount of peroxidase required. Apparent inactivation of peroxidase during phenol polymerizing reaction was found to be mainly caused by adsorption of peroxidase by polymerized phenol. Additives, such as gelatin and polyethylene glycol, suppressed this adsorption, allowing a reduction in the amount of peroxidase to 1/200. The method using gelatin as an additive was applicable to solutions containing up to 30 g/l phenol and it was successfully used to dephenolize actual wastewater containing phenol, 2-propanol and 2-butanone.