PROTOCOL FOR RESOLVING PROTEIN MIXTURES IN CAPILLARY ZONE ELECTROPHORESIS

被引：121

作者：

GORDON, MJ ^{[1
]}

LEE, KJ ^{[1
]}

ARIAS, AA ^{[1
]}

ZARE, RN ^{[1
]}

机构：

[1] STANFORD UNIV,DEPT CHEM,STANFORD,CA 94305

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 01期

关键词：

D O I：

10.1021/ac00001a012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The separation of protein mixtures by capillary zone electrophoresis can be plagued by wall adsorption of the protein components, causing peak broadening and distortion. A method is presented for overcoming this problem by adding ethylene glycol to the protein sample and by choosing the running buffer and protein sample to be at different pH values and molarities. This protocol appears to work for a wide class of proteins having different molecular weights and pI values. The method has been applied to the analysis of proteins in human serum. Compared to the traditional method of agarose gel electrophoresis, the present method is more rapid and offers better resolution, suggesting its potential as a clinical diagnosis of certain disease states.

引用

页码：69 / 72

页数：4

共 23 条

[1]

Blackburn P, 1982, ENZYMES, VXV, P317

[2] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].