CONSTITUTIVE EXPRESSION OF PORE-FORMING PROTEIN IN PERIPHERAL-BLOOD GAMMA-DELTA-T-CELLS - IMPLICATION FOR THEIR CYTOTOXIC ROLE INVIVO

被引：108
作者：
NAKATA, M
SMYTH, MJ
NORIHISA, Y
KAWASAKI, A
SHINKAI, Y
OKUMURA, K
YAGITA, H
机构：
[1] JUNTENDO UNIV, SCH MED, DEPT IMMUNOL, 2-1-1 HONGO, BUNKYO KU, TOKYO 113, JAPAN
[2] NCI, FREDERICK CANC RES & DEV CTR, EXPTL IMMUNOL LAB, FREDERICK, MD 21701 USA
来源：
JOURNAL OF EXPERIMENTAL MEDICINE | 1990年 / 172卷 / 06期
关键词：
D O I：
10.1084/jem.172.6.1877
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The cytotoxic activity and pore-forming protein (PFP) expression of human peripheral blood (PB) γ/δ T cells were examined. Fresh γ/δ T cells isolated from PB lymphocytes by fluorescenceactivated cell sorting exhibited a substantial natural killer-like cytotoxic activity against K562 target cells and had a high cytotoxic potential triggered by anti-CD3 monoclonal antibody (mAb) against P815 target cells bearing Fc γR. Immunocytochemical staining with an anti-PFP mAb revealed that virtually all PB γ/δ T cells are granular lymphocytes with abundant PFP in their cytoplasmic granules. Constitutive expression of PFP in PB 'y/6 T cells was also demonstrated by Northern blot analysis. These observations support the proposed role ofγ/δ T cells in cytolysic immune surveillance in vivo. © 1990, Rockefeller University Press., All rights reserved.
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页码：1877 / 1880
页数：4
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