SPERM GLYCOPROTEINS OF THE BOAR, BULL, RABBIT, AND RAM .2. SURFACE GLYCOPROTEINS AND FREE ACIDIC GROUPS

被引:12
作者
FLECHON, JE
机构
[1] Département de Physiologie Animale, Institut National de la Recherche Agronomique, Jouy-En-Josas
来源
GAMETE RESEARCH | 1979年 / 2卷 / 01期
关键词
farm mammals; free acidic groups; glycoproteins; plasma membrane; spermatozoa;
D O I
10.1002/mrd.1120020107
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Ejaculated spermatozoa of boars, bulls, rabbits, and rams were embedded in glycolmethacrylate and thin sections stained with phosphotungstic acid at low pH in order to observe the distribution of glycoproteins of the plasma membrane. Colloidal iron hydroxide was also used to detect the free acidic groups present on the sperm surface. Species‐specific patterns of localizations of glycoproteins and linked negative charges were observed. The distribution was sometimes homogeneous as in bull, but generally heterogeneous in the other species. The significance of the results on sperm surface components and the practical interest to know their normal distribution are discussed. Copyright © 1979 Alan R. Liss, Inc.
引用
收藏
页码:53 / 64
页数:12
相关论文
共 51 条
[1]  
Bedford J.M., 1974, Contributions Primatol, V3, P97
[2]  
Bedford J M, 1973, J Reprod Fertil Suppl, V18, P199
[3]  
BEDFORD JM, 1971, GENETICS SPERMATOZOO, P69
[4]   ULTRASTRUCTURAL CYTOCHEMISTRY OF HUMAN ADRENAL-MEDULLA [J].
BENCHIMOL, S ;
CANTIN, M .
HISTOCHEMISTRY, 1977, 54 (01) :9-26
[5]  
COURTENS JP, 1973, COMMUNICATION
[6]   SPECIFICITY OF PHOSPHOTUNGSTIC ACID USED AS A SECTION STAIN TO VISUALIZE SURFACE COATS OF CELLS [J].
DERMER, GB .
JOURNAL OF ULTRASTRUCTURE RESEARCH, 1973, 45 (3-4) :183-191
[7]   MOLECULAR PROBES OF SPERMATOZOAN STRUCTURES [J].
EDELMAN, GM ;
MILLETTE, CF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (10) :2436-&
[8]  
EDELMAN GM, 1974, FUNCTIONAL ANATOMY S, P349
[9]  
Flechon J-E, 1975, BIOL SPERMATOZOA TRA, P36
[10]  
FLECHON JE, 1975, ANN HISTOCHIM, V20, P291