DETERMINATION OF INDOLE ALKALOIDS FROM R-SERPENTINA AND R-VOMITORIA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

被引：30

作者：

KLYUSHNICHENKO, VE ^{[1
]}

YAKIMOV, SA ^{[1
]}

TUZOVA, TP ^{[1
]}

SYAGAILO, YV ^{[1
]}

KUZOVKINA, IN ^{[1
]}

WULFSON, AN ^{[1
]}

MIROSHNIKOV, AI ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,KA TIMIRYAZEV PLANT PHYSIOL INST,MOSCOW 127276,RUSSIA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 704卷 / 02期

关键词：

D O I：

10.1016/0021-9673(95)00082-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of a model mixture of six indole alkaloids by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) on the normal- and reversed-phase Armsorb supports, respectively, was studied. A procedure for the analysis of extracts from plants cell callus culture of Rauwolfia serpentina and hairy roots of R. serpentina and R. vomitoria in the presence of these alkaloids and its derivatives with different chromatographic systems was elaborated. It was found that the alkaloid compositions of plant cell cultures and hairy roots of R. serpentina and R. vomitoria are identical. With HPLC analysis a higher precision of alkaloid determination in solution and measurements of concentration is achieved. HPTLC analysis gives a qualitative identification of the extracts. The combination of these high-performance methods provides quantitative and qualitative analyses of indole alkaloids such as ajmaline, ajmalicine, reserpine, raucaffricine, serpentine and yohimbine.

引用

页码：357 / 362

页数：6

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