RAPID DETECTION OF PCR PRODUCTS OF HEPATITIS-C VIRUS (HCV) WITH A NONRADIOACTIVE OLIGOPROBE

被引：0
|
作者：
CANEPARI, P
DISTEFANO, CF
LLEO, MM
机构：
来源：
MICROBIOLOGICA | 1994年 / 17卷 / 01期
关键词：
PCR; HCV; PROBE; DIGOXIGENIN;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Dot-blot for rapid detection of PCR amplification products of hepatitis C virus (HCV) using a digoxigenin (DIG)-labelled oligoprobe was developed and its sensitivity compared with Southern blot hybridization. The specificity and sensitivity of the DIG-labelled probe were identical to those of the 32P-labelled when the DIG-labelled nucleic acids were detected by enzyme-catalyzed chemiluminescent reaction. The lack of radioactivity makes this procedure suitable for routine use in diagnostic laboratories.
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页码：9 / 14
页数：6
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