RAPID DETECTION OF PCR PRODUCTS OF HEPATITIS-C VIRUS (HCV) WITH A NONRADIOACTIVE OLIGOPROBE

被引：0
|
作者：
CANEPARI, P
DISTEFANO, CF
LLEO, MM
机构：
来源：
MICROBIOLOGICA | 1994年 / 17卷 / 01期
关键词：
PCR; HCV; PROBE; DIGOXIGENIN;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Dot-blot for rapid detection of PCR amplification products of hepatitis C virus (HCV) using a digoxigenin (DIG)-labelled oligoprobe was developed and its sensitivity compared with Southern blot hybridization. The specificity and sensitivity of the DIG-labelled probe were identical to those of the 32P-labelled when the DIG-labelled nucleic acids were detected by enzyme-catalyzed chemiluminescent reaction. The lack of radioactivity makes this procedure suitable for routine use in diagnostic laboratories.
引用
收藏
页码：9 / 14
页数：6
相关论文
共 50 条
  • [1] DETECTION OF HEPATITIS-C VIRUS (HCV) RNA BY PCR RELATED TO HCV ANTIBODIES IN SERUM AND LIVER HISTOLOGY IN SWEDISH BLOOD-DONORS
    YUN, ZB
    LINDH, G
    WEILAND, O
    JOHANSSON, B
    SONNERBORG, A
    JOURNAL OF MEDICAL VIROLOGY, 1993, 39 (01) : 57 - 61
  • [2] EPIDEMIOLOGY OF HEPATITIS-C VIRUS (HCV)
    DELAPORTE, E
    DAZZA, MC
    LAROUZE, B
    MEDECINE ET MALADIES INFECTIEUSES, 1995, 25 : 1084 - 1088
  • [3] REPLICATIVE LEVEL OF HEPATITIS-C VIRUS (HCV) IN CHRONIC HEPATITIS-C
    HAYASHI, N
    KAMADA, T
    INTERNAL MEDICINE, 1993, 32 (12) : 944 - 945
  • [4] CLINICAL-SIGNIFICANCE OF HEPATITIS-C VIRUS (HCV) INFECTION IN LIVER-TRANSPLANT RECIPIENTS - ROLE OF SEROLOGY AND HCV RNA DETECTION
    LUMBRERAS, C
    DELGADO, R
    FUERTES, A
    LOINAZ, C
    IGLESIAS, J
    COLINA, F
    AGUADO, JM
    GIMENO, C
    GARCIA, I
    LIZASOAIN, M
    MORENO, E
    NORIEGA, AR
    DIGESTIVE DISEASES AND SCIENCES, 1994, 39 (05) : 965 - 969
  • [5] DETECTION OF HEPATITIS-C VIRUS-RNA IN THE LIVER BY IN-SITU HYBRIDIZATION
    TANAKA, Y
    ENOMOTO, N
    KOJIMA, S
    TANG, L
    GOTO, M
    MARUMO, F
    SATO, C
    LIVER, 1993, 13 (04): : 203 - 208
  • [6] DETECTION OF HEPATITIS-C VIRUS (HCV) RNA SEQUENCES IN LIVER-TISSUE BY INSITU HYBRIDIZATION
    LAMAS, E
    BACCARINI, P
    HOUSSET, C
    KREMSDORF, D
    BRECHOT, C
    JOURNAL OF HEPATOLOGY, 1992, 16 (1-2) : 219 - 223
  • [7] RAPID DETECTION OF HEPATITIS-C VIRUS-RNA BY DIRECT CAPTURE FROM BLOOD
    VANDOORN, LJ
    KLETER, B
    VOERMANS, J
    MAERTENS, G
    BROUWER, H
    HEIJTINK, R
    QUINT, W
    JOURNAL OF MEDICAL VIROLOGY, 1994, 42 (01) : 22 - 28
  • [8] Detection of genotype 4 Hepatitis C virus (HCV) in Italy
    Tagliaferro, L
    Corbelli, M
    Crovatto, M
    Santini, GF
    MICROBIOLOGICA, 1997, 20 (01): : 87 - 88
  • [9] DETECTION OF HEPATITIS-C VIRUS BY POLYMERASE CHAIN-REACTION IN HEMODIALYZED PATIENTS IN RELATIONSHIP TO ANTI-HCV STATUS
    BOUCHARDEAU, F
    CHAUVEAU, P
    LEMARREC, N
    GIRAULT, A
    ZINS, B
    COUROUCE, AM
    RESEARCH IN VIROLOGY, 1993, 144 (03): : 233 - 242
  • [10] A SIMPLE SINGLE-TUBE PROCEDURE OF PCR ASSAY FOR THE DETECTION OF HEPATITIS-C VIRUS-RNA
    HAYASHI, Y
    KANEKO, S
    JOMORI, T
    KOBARA, Y
    SUZUKI, E
    TASAKA, F
    MURAKAMI, S
    KOBAYASHI, K
    MITANI, T
    RESEARCH IN VIROLOGY, 1994, 145 (02): : 123 - 128
12345