THE ASSAY OF CLOZAPINE AND N-DESMETHYLCLOZAPINE IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：62
作者：
LOVDAHL, MJ [1 ]
PERRY, PJ [1 ]
MILLER, DD [1 ]
机构：
[1] UNIV IOWA,COLL MED,DEPT PSYCHIAT,500 NEWTON RD,IOWA CITY,IA 52242
来源：
THERAPEUTIC DRUG MONITORING | 1991年 / 13卷 / 01期
关键词：
CLOZAPINE; DESMETHYLCLOZAPINE; ASSAY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;
D O I：
10.1097/00007691-199101000-00010
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
A high performance liquid chromatographic method for the simulataneous quantitation of clozapine-a member of the dibenzodiazepine class of antipsychotic drugs-and its reduced metabolite in human plasma has been developed. Clozapine and N-desmethylclozapine are concentrated from blood samples by liquid-liquid extraction, followed by reverse-phase liquid chromatography. Accuracy of the determination is ensured by application of an internal standard, protriptyline, which is closely related to clozapine. A detection limit of 15 ng/mL for clozapine and 30 ng/ml for N-desmethylclozapine was achieved.
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页码：69 / 72
页数：4
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