THE ASSAY OF CLOZAPINE AND N-DESMETHYLCLOZAPINE IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：62

作者：

LOVDAHL, MJ ^{[1
]}

PERRY, PJ ^{[1
]}

MILLER, DD ^{[1
]}

机构：

[1] UNIV IOWA,COLL MED,DEPT PSYCHIAT,500 NEWTON RD,IOWA CITY,IA 52242

来源：

THERAPEUTIC DRUG MONITORING | 1991年 / 13卷 / 01期

关键词：

CLOZAPINE; DESMETHYLCLOZAPINE; ASSAY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;

D O I：

10.1097/00007691-199101000-00010

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A high performance liquid chromatographic method for the simulataneous quantitation of clozapine-a member of the dibenzodiazepine class of antipsychotic drugs-and its reduced metabolite in human plasma has been developed. Clozapine and N-desmethylclozapine are concentrated from blood samples by liquid-liquid extraction, followed by reverse-phase liquid chromatography. Accuracy of the determination is ensured by application of an internal standard, protriptyline, which is closely related to clozapine. A detection limit of 15 ng/mL for clozapine and 30 ng/ml for N-desmethylclozapine was achieved.

引用

页码：69 / 72

页数：4

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