DIFFERENTIATION OF CYTOMEGALOVIRUS STRAINS BY RESTRICTION ANALYSIS OF DNA-SEQUENCES AMPLIFIED FROM CLINICAL SPECIMENS

被引：85
作者：
CHOU, SW
机构：
[1] OREGON HLTH SCI UNIV,DIV INFECT DIS,PORTLAND,OR 97201
[2] VET ADM MED CTR,RES SERV,PORTLAND,OR 97207
来源：
JOURNAL OF INFECTIOUS DISEASES | 1990年 / 162卷 / 03期
关键词：
D O I：
10.1093/infdis/162.3.738
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
DNA sequences of 2.0 to 2.7 kb were amplified by polymerase chain reaction from four genes (major immediate-early, DNA polymerise, and glycoproteins B and H) of 20 distinct strains of cytomegalovirus (CMV). Analysis of the unpurified reaction products using selected restriction enzymes showed sufficient diversity of profiles among strains to differentiate all 20. Sequences suitable for restriction analysis were also amplified directly from clinical urine specimens and showed restriction profiles comparable to those of sequences amplified from corresponding CMV culture isolates. Ability to identify specific strains of CMV in amplification products facilitates molecular epidemiologic studies. © 1990, by The University of Chicago.
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页码：738 / 742
页数：5
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