EFFECT OF 6-HYDROXYDOPAMINE ON MURINE HEMATOPOIETIC STEM-CELLS - ENHANCED CYTOTOXICITY ON MEGAKARYOCYTE COLONY-FORMING-UNITS

被引：7

作者：

GORDON, BG ^{[1
]}

DEBOER, JM ^{[1
]}

WOOLDRIDGE, LD ^{[1
]}

SHARP, JG ^{[1
]}

机构：

[1] UNIV NEBRASKA,MED CTR,DEPT ANAT,OMAHA,NE 68105

来源：

LIFE SCIENCES | 1991年 / 49卷 / 02期

关键词：

D O I：

10.1016/0024-3205(91)90025-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We examined the effect of catecholaminergic neurotoxin 6-hydroxydopamine (6-OHDA) on murine committed megakaryocyte progenitor cells, the megakaryocyte-colony forming unit (CFU-Meg). More mature cells of the megakaryocyte series have the capacity for active uptake of catecholamines, and we speculated that the CFU-Meg would also take up 6-OHDA and be selectively killed. CFU-Meg were much more sensitive to the effects of this agent than were granulocyte-macrophage colony forming units (CFU-GM) or spleen-colony forming units. Co-incubation with catalase, which would destroy hydrogen peroxide generated extracellularly by the autoxidation of 6-OHDA, ablated 6-OHDA toxicity towards CFU-GM, but also significantly reduced the effect on CFU-Meg. Mazindol, a selective dopamine uptake inhibitor did not alter 6-OHDA effect on either CFU-Meg or CFU-GM. Finally, CFU-Meg were no more sensitive to incubation with hydrogen peroxide than were CFU-GM. These data suggest that CFU-Meg, unlike their more mature progeny, do not actively concentrate 6-OHDA, and the excess toxicity of this agnet towards CFU-Meg is probably due to increased sensitivity to autoxidation products of 6-OHDA, other than hydrogen peroxide, generated extracellularly.

引用

页码：121 / 127

页数：7

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[3]

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