MOLECULAR-CLONING AND EXPRESSION OF A DELTA-OPIOID RECEPTOR FROM RAT-BRAIN

被引：46
作者：
ABOOD, ME
NOEL, MA
FARNSWORTH, JS
TAO, Q
机构：
[1] Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonweath University, Richmond
来源：
JOURNAL OF NEUROSCIENCE RESEARCH | 1994年 / 37卷 / 06期
关键词：
RAT; CDNA-BRAIN; DOR-1;
D O I：
10.1002/jnr.490370605
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We have isolated and characterized a rat delta-opioid receptor. The deduced amino acid sequence (372 aa) closely resembles the murine delta-opioid receptor, DOR-1. In fact, 97% of the amino acid residues are conserved between the two species, while 93% of the nucleic acid residues are identical. A 6 kb mRNA was detected in rat cortex using rat DOR-1 as a probe. When expressed in COS cells, the clone shows high affinity opioid binding with selectivity for delta-opioids. The rat delta-opioid receptor cDNA clone will be a useful tool for studying the function of delta-opioid receptor in rats. (C) 1994 Wiley-Liss, Inc.
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收藏
页码：714 / 719
页数：6
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