PREPARATION OF AN AFFINITY RESIN FOR ODORANTS BY COUPLING ODORANT BINDING-PROTEIN FROM BOVINE NASAL-MUCOSA TO SEPHAROSE-4B

被引：8

作者：

BUSSOLATI, L ^{[1
]}

RAMONI, R ^{[1
]}

GROLLI, S ^{[1
]}

DONOFRIO, G ^{[1
]}

BIGNETTI, E ^{[1
]}

机构：

[1] UNIV PARMA,INST VET BIOCHEM,V TAGLIO 8,I-43100 PARMA,ITALY

来源：

JOURNAL OF BIOTECHNOLOGY | 1993年 / 30卷 / 02期

关键词：

ODORANT-BINDING PROTEIN; ODORANT; AFFINITY CHROMATOGRAPHY;

D O I：

10.1016/0168-1656(93)90115-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The soluble Odorant Binding protein (OBP) of bovine nasal mucosa is a dimer of non-covalently bound subunits that specifically recognizes several odorants of different chemical classes. Monomerization and binding activity impairment are concomitant reversible processes that occur below pH 6.0. In order to avoid this inconvenience, a mild pH condition has been chosen to couple native OBP to CNBr-activated Sepharose 4B resin. Above pH 6.0, the OBP-Sepharose matrix shows high specificity and affinity for odorants, thus opening new perspectives in odorant-sensor technology.

引用

页码：225 / 230

页数：6

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