ADRENOMEDULLIN STIMULATES CYCLIC-AMP FORMATION IN RAT VASCULAR SMOOTH-MUSCLE CELLS

被引：324

作者：

ISHIZAKA, Y ^{[1
]}

TANAKA, M ^{[1
]}

KITAMURA, K ^{[1
]}

KANGAWA, K ^{[1
]}

MINAMINO, N ^{[1
]}

MATSUO, H ^{[1
]}

ETO, T ^{[1
]}

机构：

[1] NATL CARDIOVASC CTR, RES INST, SUITA, OSAKA 565, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1496

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC). The EC(50) value of human AM is 2 X 10(-8)M which is slightly higher than that of CGRP (8.5 X 10(-9)M). In a receptor binding assay for AM in rat VSMC, the binding of [I-125] AM was competitively inhibited by human AM, but not by human CGRP. Thus, AM is thought to increase intracellular cAMP in rat VSMC via its specific receptor to evoke vasodilation. (C) 1994 Academic Press, Inc.

引用

页码：642 / 646

页数：5

共 12 条

[1]

BRAIN SD, 1984, NATURE, P54

[2]

CHAMLEY JH, 1977, CELL TISSUE RES, V177, P503

[3] AMYLIN OR CGRP (8-37) FRAGMENTS REVERSE AMYLIN-INDUCED INHIBITION OF C-14 GLYCOGEN ACCUMULATION [J].

DEEMS, RO ;

CARDINAUX, F ;

DEACON, RW ;

YOUNG, DA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 181 (01) :116-120

[4] INHIBITION OF PERIARTERIAL NERVE STIMULATION-INDUCED VASODILATION OF THE MESENTERIC ARTERIAL BED BY CGRP (8-37) AND CGRP RECEPTOR DESENSITIZATION [J].

HAN, SP ;

NAES, L ;

WESTFALL, TC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 168 (02) :786-791

[5] HEMODYNAMIC-EFFECTS OF A NOVEL HYPOTENSIVE PEPTIDE, HUMAN ADRENOMEDULLIN, IN RATS [J].

ISHIYAMA, Y ;

KITAMURA, K ;

ICHIKI, Y ;

NAKAMURA, S ;

KIDA, O ;

KANGAWA, K ;

ETO, T .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1993, 241 (2-3) :271-273

[6] ISOLATION AND CHARACTERIZATION OF PEPTIDES WHICH ACT ON RAT PLATELETS, FROM A PHEOCHROMOCYTOMA [J].

KITAMURA, K ;

KANGAWA, K ;

KAWAMOTO, M ;

ICHIKI, Y ;

MATSUO, H ;

ETO, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 185 (01) :134-141

[7] ADRENOMEDULLIN - A NOVEL HYPOTENSIVE PEPTIDE ISOLATED FROM HUMAN PHEOCHROMOCYTOMA [J].

KITAMURA, K ;

KANGAWA, K ;

KAWAMOTO, M ;

ICHIKI, Y ;

NAKAMURA, S ;

MATSUO, H ;

ETO, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 192 (02) :553-560

[8] CALCITONIN GENE-RELATED PEPTIDE STIMULATES CYCLIC-AMP FORMATION IN RAT AORTIC SMOOTH-MUSCLE CELLS [J].

KUBOTA, M ;

MOSELEY, JM ;

BUTERA, L ;

DUSTING, GJ ;

MACDONALD, PS ;

MARTIN, TJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 132 (01) :88-94

[9] ISOLATION AND CHARACTERIZATION OF HUMAN CALCITONIN GENE-RELATED PEPTIDE [J].

MORRIS, HR ;

PANICO, M ;

ETIENNE, T ;

TIPPINS, J ;

GIRGIS, SI ;

MACINTYRE, I .

NATURE, 1984, 308 (5961) :746-748

[10]

NUKI C, 1993, BIOCHEM BIOPH RES CO, V192, P553

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