ASSOCIATION OF N-GLYCOSYLATION OF APOLIPOPROTEIN-B-100 WITH PLASMA-CHOLESTEROL LEVELS IN WATANABE HERITABLE HYPERLIPIDEMIC RABBITS

被引：4

作者：

TSUNEMITSU, M ^{[1
]}

ISHIKAWA, Y ^{[1
]}

TANIGUCHI, T ^{[1
]}

FUKUZAKI, H ^{[1
]}

YOKOYAMA, M ^{[1
]}

机构：

[1] KOBE UNIV,SCH MED,DEPT INTERNAL MED 1,7-5-1 KUSUNOKI CHO,CHUO KU,KOBE 650,JAPAN

来源：

ATHEROSCLEROSIS | 1992年 / 93卷 / 03期

关键词：

N-GLYCOSYLATION; N-LINKED SUGAR CHAIN; APOLIPOPROTEIN-B-100; WATANABE HERITABLE HYPERLIPIDEMIC (WHHL) RABBIT; CHOLESTEROL;

D O I：

10.1016/0021-9150(92)90259-J

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have previously demonstrated the heterogeneity of N-linked sugar chains of apolipoprotein (apo) B-100 in Watanabe heritable hyperlipidemic (WHHL) rabbit and fasting Japanese White rabbits (Arteriosclerosis, 10 (1990) 386-393). To investigate further the role of N-linked sugar chains of apo B-100 in lipid metabolism, we examined the correlation between the N-glycosylation of apo B-100 and serum cholesterol levels in WHHL rabbits. The N-linked sugar chains of apo B-100 were liberated by hydrazinolysis, followed by NaB3H4 reduction and were fractionated by paper electrophoresis and Bio-Gel P-4 column chromatography. These were found to consist of one neutral (N) and two acidic fractions (A1 and A2). N contained a high mannose type oligosaccharide consisting of Man5 . GlcNAc2 to Man9 . GlcNAc2, while A1 and A2 contained monosialylated and disialylated complex type oligosaccharides, respectively. The molar ratio varied among the 5 WHHL rabbits. There was an inverse correlation between the ratio of acidic oligosaccharide fractions (A1 + A2) and serum cholesterol levels (r = -0.971, P < 0.01) in the 5 WHHL rabbits. These results indicate that the N-glycosylation of apo B-100 is closely related to cholesterol metabolism in WHHL rabbits.

引用

页码：229 / 235

页数：7