Determination of Total Phenolic, Flavonoid Content and Antioxidant Activity of Terminalia Chebula (Fruit).

The antioxidant activity of methanolic extract of Terminalia chebula was evaluated concerning total phenol and flavonoid contents. Three commonly in vitro methods are used to assess antioxidant activity namely Ferric reducing antioxidant power (FRAP assay), DPPH Free radical scavenging ability assay. The results showed that the total phenolic content for 50, 100 and 200 mu g/ml extracts were 0.2232, 0.3156 and 0.4170 mg GAE/gram respectively. The total flavonoid content for 50, 100 and 200 mu g/ml extracts were 49, 63, and 83 mg RE/gram respectively. In the DPPH study, the plant extract shown appreciable anti-oxidant potency as the percentage of anti- oxidant activity is more significant compared to the standard, BHT. In the FRAP study, the values of absorbance recorded for both standard and test samples at a concentration of 400 mu g/ml are 4.5000 and 3.4889 respectively. From these results, the presence studies indicate that methanolic extract Terminalia chebula might have good potential as a source for natural health products due to its antioxidant activities.