MOLECULAR-CLONING OF CDNA FOR THE CARCINOMA-ASSOCIATED ANTIGEN GA733-2

被引：165

作者：

SZALA, S ^{[1
]}

FROEHLICH, M ^{[1
]}

SCOLLON, M ^{[1
]}

KASAI, Y ^{[1
]}

STEPLEWSKI, Z ^{[1
]}

KOPROWSKI, H ^{[1
]}

LINNENBACH, AJ ^{[1
]}

机构：

[1] WISTAR INST,3601 SPRUCE ST,PHILADELPHIA,PA 19104

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 09期

关键词：

Antigen GA733-1; COS cell expression system; Monoclonal antibody;

D O I：

10.1073/pnas.87.9.3542

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Defined by monoclonal antibody GA733, the GA733-2 antigen is a cell surface 40-kDa glycoprotein associated with human carcinomas of various origins. Molecular clones for the GA733-2 antigen were isolated from a colorectal carcinoma cell line cDNA library using the high-efficiency COS cell expression system, A 1.4-kilobase cDNA species was enriched by immunoselection with monoclonal antibody. The authenticity of individual clones was established by immunologic and sequence criteria. At the amino acid sequence level, GA733-2 was found to be >99% identical to the previously described KSA antigen defined by monoclonal antibody KS1/4. The amino acid sequence derived from the previously described GA733-related gene, GA733-1, was found to be 49% identical to GA733-2. The positions of 12 cysteine residues in the extracellular domains of the two GA733 antigens are conserved, as is the overall distribution of Hydrophobic and hydrophilic residues. A 1.45-kilobase transcript of the GA733-2/KSA gene was found to be expressed in cell lines derived from colorectal and pancreatic carcinoma.

引用

页码：3542 / 3546

页数：5

共 32 条

[1] MOLECULAR-CLONING OF A CD28 CDNA BY A HIGH-EFFICIENCY COS CELL EXPRESSION SYSTEM [J].