Genome Resequencing of the Virulent and Multidrug-Resistant Reference Strain Clostridium difficile 630

被引:26
作者
Riedel, Thomas [1 ]
Bunk, Boyke [1 ,2 ]
Thuermer, Andrea [3 ,4 ]
Sproeer, Cathrin [1 ]
Brzuszkiewicz, Elzbieta [3 ,4 ]
Abt, Birte [1 ,2 ]
Gronow, Sabine [1 ,2 ]
Liesegang, Heiko [3 ,4 ]
Daniel, Rolf [3 ,4 ]
Overmann, Joerg [1 ,2 ]
机构
[1] Leibniz Inst DSMZ German Collect Microorganisms &, Braunschweig, Germany
[2] German Ctr Infect Res DZIF, Partner Site Hannover Braunschweig, Braunschweig, Germany
[3] Georg August Univ Gottingen, Dept Genom & Appl Microbiol, Gottingen, Germany
[4] Georg August Univ Gottingen, Gottingen Genom Lab, Gottingen, Germany
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D O I
10.1128/genomeA.00276-15
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学科分类号
摘要
We resequenced the complete genome of the virulent and multidrug-resistant pathogen Clostridium difficile strain 630. A combination of single-molecule real-time and Illumina sequencing technology revealed the presence of an additional rRNA gene cluster, additional tRNAs, and the absence of a transposon in comparison to the published and reannotated genome sequence.
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共 6 条
[1]   Fast and accurate long-read alignment with Burrows-Wheeler transform [J].
Li, Heng ;
Durbin, Richard .
BIOINFORMATICS, 2010, 26 (05) :589-595
[2]   Reannotation of the genome sequence of Clostridium difficile strain 630 [J].
Monot, Marc ;
Boursaux-Eude, Caroline ;
Thibonnier, Marie ;
Vallenet, David ;
Moszer, Ivan ;
Medigue, Claudine ;
Martin-Verstraete, Isabelle ;
Dupuy, Bruno .
JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (08) :1193-1199
[3]   The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome [J].
Sebaihia, Mohammed ;
Wren, Brendan W. ;
Mullany, Peter ;
Fairweather, Neil F. ;
Minton, Nigel ;
Stabler, Richard ;
Thomson, Nicholas R. ;
Roberts, Adam P. ;
Cerdeno-Tarrraga, Ana M. ;
Wang, Hongmei ;
Holden, Matthew T. G. ;
Wright, Anne ;
Churcher, Carol ;
Quail, Michael A. ;
Baker, Stephen ;
Bason, Nathalie ;
Brooks, Karen ;
Chillingworth, Tracey ;
Cronin, Ann ;
Davis, Paul ;
Dowd, Linda ;
Fraser, Audrey ;
Feltwell, Theresa ;
Hance, Zahra ;
Holroyd, Simon ;
Jagels, Kay ;
Moule, Sharon ;
Mungall, Karen ;
Price, Claire ;
Rabbinowitsch, Ester ;
Sharp, Sarah ;
Simmonds, Mark ;
Stevens, Kim ;
Unwin, Louise ;
Whithead, Sally ;
Dupuy, Bruno ;
Dougan, Gordon ;
Barrell, Bart ;
Parkhill, Julian .
NATURE GENETICS, 2006, 38 (07) :779-786
[4]   Prokka: rapid prokaryotic genome annotation [J].
Seemann, Torsten .
BIOINFORMATICS, 2014, 30 (14) :2068-2069
[5]   Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5 [J].
van Eijk, Erika ;
Anvar, Seyed Yahya ;
Browne, Hilary P. ;
Leung, Wai Yi ;
Frank, Jeroen ;
Schmitz, Arnoud M. ;
Roberts, Adam P. ;
Smits, Wiep Klaas .
BMC GENOMICS, 2015, 16
[6]  
WUST J, 1982, J CLIN MICROBIOL, V16, P1096