REPETITIVE SEQUENCES IN THE RIBOSOMAL INTERGENIC SPACER OF TRYPANOSOMA-CRUZI

被引：26

作者：

NOVAK, EM

DEMELLO, MP

GOMES, HBM

GALINDO, I

GUEVARA, P

RAMIREZ, JL

DASILVERIA, JF

机构：

[1] ESCOLA PAULISTA MED,DISCIPLINA PARASITOL,RUA BOTUCATU 862,6 ANDAR,BR-04023-062 SAO PAULO,BRAZIL

[2] CENT UNIV VENEZUELA,DEPT BIOL CELULAR,CARACAS,VENEZUELA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 60卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

TRYPANOSOMA-CRUZI; RIBOSOMAL INTERGENIC SPACER; SPACER REPETITIVE ELEMENTS; SPECIES-SPECIFIC SEQUENCE; CHI-LIKE SEQUENCE;

D O I：

10.1016/0166-6851(93)90138-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fragment of Trypanosoma cruzi ribosomal intergenic spacer (IGS) located at 6.7 kb from the 3' end of the 24S rRNA gene was analyzed. This IGS fragment is characterized by the presence of three types of repetitive elements (designated Spacer Repetitive Elements, SRE), short direct repeats (5-6 bp) and chi-like recombinational sequences. SRE elements are composed of relatively short repeats (43-145 bp) which show variabilities consisting of nucleotide changes, insertions and deletions. SRE-1 element (145 bp) has a short oligo(dA) tail at the end of the repeat and can be found flanked by other SRE elements. SRE elements are species-specific, suggesting that probes based on them may be diagnostic for Trypanosoma cruzi.

引用

页码：273 / 280

页数：8

共 29 条

[11] LAMBDA-REPLACEMENT VECTORS CARRYING POLYLINKER SEQUENCES [J].