REPETITIVE SEQUENCES IN THE RIBOSOMAL INTERGENIC SPACER OF TRYPANOSOMA-CRUZI

被引：26
作者：
NOVAK, EM
DEMELLO, MP
GOMES, HBM
GALINDO, I
GUEVARA, P
RAMIREZ, JL
DASILVERIA, JF
机构：
[1] ESCOLA PAULISTA MED,DISCIPLINA PARASITOL,RUA BOTUCATU 862,6 ANDAR,BR-04023-062 SAO PAULO,BRAZIL
[2] CENT UNIV VENEZUELA,DEPT BIOL CELULAR,CARACAS,VENEZUELA
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 60卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
TRYPANOSOMA-CRUZI; RIBOSOMAL INTERGENIC SPACER; SPACER REPETITIVE ELEMENTS; SPECIES-SPECIFIC SEQUENCE; CHI-LIKE SEQUENCE;
D O I：
10.1016/0166-6851(93)90138-N
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A fragment of Trypanosoma cruzi ribosomal intergenic spacer (IGS) located at 6.7 kb from the 3' end of the 24S rRNA gene was analyzed. This IGS fragment is characterized by the presence of three types of repetitive elements (designated Spacer Repetitive Elements, SRE), short direct repeats (5-6 bp) and chi-like recombinational sequences. SRE elements are composed of relatively short repeats (43-145 bp) which show variabilities consisting of nucleotide changes, insertions and deletions. SRE-1 element (145 bp) has a short oligo(dA) tail at the end of the repeat and can be found flanked by other SRE elements. SRE elements are species-specific, suggesting that probes based on them may be diagnostic for Trypanosoma cruzi.
引用
收藏
页码：273 / 280
页数：8
相关论文
共 29 条
[1]   MOUSE RDNA NON-TRANSCRIBED SPACER SEQUENCES ARE FOUND FLANKING IMMUNOGLOBULIN-CH GENES AND ELSEWHERE THROUGHOUT THE GENOME [J].
ARNHEIM, N ;
SEPERACK, P ;
BANERJI, J ;
LANG, RB ;
MIESFELD, R ;
MARCU, KB .
CELL, 1980, 22 (01) :179-185
[2]  
ARRUDA M V D, 1990, Molecular and Biochemical Parasitology, V40, P35
[3]   TRYPANOSOMA-CRUZI RIBOSOMAL-RNA - INTERNAL BREAK IN THE LARGE-MOLECULAR MASS SPECIES AND NUMBER OF GENES [J].
CASTRO, C ;
HERNANDEZ, R ;
CASTANEDA, M .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1981, 2 (3-4) :219-233
[4]   GENOME ORGANIZATION AND PLOIDY NUMBER IN TRYPANOSOMA-CRUZI [J].
CASTRO, C ;
CRAIG, SP ;
CASTANEDA, M .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1981, 4 (5-6) :273-282
[5]   CHARACTERIZATION OF A TRYPANOSOMA-CRUZI GENOMIC FRAGMENT COMPLEMENTARY TO SEVERAL SPECIES-SPECIFIC MESSENGER-RNAS BUT DIFFERENT FROM THE SPLICED LEADER SEQUENCE [J].
COTRIM, PC ;
CUMMINGS, LM ;
DASILVEIRA, JF .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1989, 84 (02) :143-149
[6]  
DALESSANDRO A, 1976, BIOL KINETOPLASTIDA, V1, P328
[7]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[8]   A COMPLEX ARRAY OF SEQUENCES ENHANCES RIBOSOMAL TRANSCRIPTION IN XENOPUS-LAEVIS [J].
DEWINTER, RFJ ;
MOSS, T .
JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (04) :813-827
[9]   HUMAN RIBOSOMAL DNA - CONSERVED SEQUENCE ELEMENTS IN A 4.3-KB REGION DOWNSTREAM FROM THE TRANSCRIPTION UNIT [J].
DICKSON, KR ;
BRAATEN, DC ;
SCHLESSINGER, D .
GENE, 1989, 84 (01) :197-200
[10]   RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS IN THE RIBOSOMAL GENE SPACERS OF TRYPANOSOMA-CRUZI AND TRYPANOSOMA-CONORHINI [J].
DIETRICH, P ;
DUSSAN, MD ;
FLOETERWINTER, LM ;
AFFONSO, MHT ;
CAMARGO, EP ;
SOARES, MB .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 42 (01) :13-19
123