PHOTOCHEMICAL REGENERATION OF NADPH USING THE ENZYME FERREDOXIN-NADP+ REDUCTASE

被引：6

作者：

PUEYO, JJ ^{[1
]}

GOMEZMORENO, C ^{[1
]}

机构：

[1] UNIV ZARAGOZA,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC & CELULAR,E-50009 ZARAGOZA,SPAIN

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1992年 / 14卷 / 01期

关键词：

PHOTOPRODUCTION; NADPH; FNR; FERREDOXIN;

D O I：

10.1016/0141-0229(92)90018-J

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ferredoxin-NADP+ reductase and the electron transfer protein ferredoxin (or flavodoxin) were used to photochemically regenerate NADPH. The presence of ferredoxin enhanced the rate of NADPH production almost fivefold when either proflavin or 5-deazariboflavin was used as photosensitizer. The very high turnover numbers (8,100 min-1) obtained for the enzyme in this system indicate the efficiency of the process. The system was also shown to be very stable, since total turnover numbers of approximately 3.2 x 10(5) were obtained during continuous operation.

引用

页码：8 / 12

页数：5

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