CLONING AND CHARACTERIZATION OF THE GENE ENCODING RABBIT CARDIAC CALSEQUESTRIN

被引：44
作者：
ARAI, M
ALPERT, NR
PERIASAMY, M
机构：
[1] UNIV VERMONT,COLL MED,DEPT PHYSIOL & BIOPHYS,BURLINGTON,VT 05403
[2] UNIV VERMONT,COLL MED,DEPT MICROBIOL & MOLEC GENET,BURLINGTON,VT 05403
来源：
GENE | 1991年 / 109卷 / 02期
关键词：
GENE EXPRESSION; MYOGENESIS; MYOGENIN; NUCLEOTIDE SEQUENCING; RECOMBINANT DNA;
D O I：
10.1016/0378-1119(91)90621-H
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A cDNA encoding rabbit cardiac calsequestrin was isolated and characterized. The deduced nascent cardiac calsequestrin contains 409 amino acids of which 26% are acidic residues, and had 93% and 67% aa identity with canine cardiac calsequestrin and rabbit fast-twitch skeletal muscle calsequestrin, respectively. RNA blot analyses indicate that this mRNA is expressed in atrium, ventricle and to a lesser amount in slow-twitch skeletal muscle. This mRNA transcript is not expressed in adult fast-twitch skeletal muscle, smooth muscle, or nonmuscle tissues. Analysis of in vitro skeletal muscle myogenesis using a mouse myoblast cell line C2C12, demonstrates that both cardiac and skeletal calsequestrin isoforms are coproduced during muscle differentiation.
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页码：275 / 279
页数：5
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