ABNORMAL-PLANT DEVELOPMENT AND DOWN-REGULATION OF PHENYLPROPANOID BIOSYNTHESIS IN TRANSGENIC TOBACCO CONTAINING A HETEROLOGOUS PHENYLALANINE AMMONIA-LYASE GENE

被引：263
作者：
ELKIND, Y
EDWARDS, R
MAVANDAD, M
HEDRICK, SA
RIBAK, O
DIXON, RA
LAMB, CJ
机构：
[1] SALK INST BIOL STUDIES, PLANT BIOL LAB, 10010 N TORREY PINES RD, LA JOLLA, CA 92037 USA
[2] HEBREW UNIV JERUSALEM, FAC AGR, IL-76100 REHOVOT, ISRAEL
[3] SAMUEL ROBERTS NOBLE FDN INC, DIV PLANT BIOL, ARDMORE, OK 73402 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 22期
关键词：
gene expression; gene transfer; phenylpropanoid products; transgenic plants;
D O I：
10.1073/pnas.87.22.9057
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Biosynthesis of phenylpropanoid natural products in tobacco was perturbed by introduction of a heterologous (bean) phenylalanine ammonia-lyase (PAL; L-phenylalanine ammonia-lyase, EC 4.3.1.5) gene, modified by inclusion of cauliflower mosaic virus 35S enhancer sequences in its promoter. These transgenic plants can exhibit a series of unusual phenotypes including localized fluorescent lesions, altered leaf shape and texture, reduced lignification in xylem, stunted growth, reduced pollen viability, and altered flower morphology and pigmentation. Genetic analysis of a transformant with severe symptoms showed that symptom development was inherited as a single, partially dominant trait and cosegregated with reduced levels of PAL activity and soluble phenylpropanoid products. Accumulation of transcripts encoded by the endogenous tobacco PAL genes was suppressed. We conclude that the transgene disrupts PAL regulation and that some of the phenotypes reflect interference with putative signals dependent on phenylpropanoid biosynthesis.
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收藏
页码：9057 / 9061
页数：5
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