MELATONIN MODIFIES CALMODULIN CELL LEVELS IN MDCK AND N1E-115 CELL-LINES AND INHIBITS PHOSPHODIESTERASE ACTIVITY INVITRO

被引：89

作者：

BENITEZKING, G ^{[1
]}

HUERTODELGADILLO, L ^{[1
]}

ANTONTAY, F ^{[1
]}

机构：

[1] UNIV AUTONOMA METROPOLITANA IZTAPALAPA,DIV CIENCIAS BIOL & SALUD,DEPT BIOL REPROD,MEXICO CITY,DF,MEXICO

来源：

BRAIN RESEARCH | 1991年 / 557卷 / 1-2期

关键词：

MELATONIN; CALMODULIN; MDCK; N1E-115; MECHANISM OF ACTION;

D O I：

10.1016/0006-8993(91)90146-M

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The interaction between melatonin and calmodulin was explored. Calmodulin cell levels in MDCK and N1E-115 cells cultured with 10(-9) M melatonin were increased after 3 days but decreased after 6 days. Melatonin inhibited calmodulin-dependent phosphodiesterase and when either melatonin or [H-3]melatonin was preincubated with calmodulin and separated by electrophoresis, comigration of calmodulin with the radioactivity as well as modification of the Ca2+ calmodulin shift were observed. The results point out that one of the mechanisms of action of melatonin is a calmodulin-melatonin interaction.

引用

页码：289 / 292

页数：4

共 20 条

[1]

Anton-Tay F, 1974, Adv Biochem Psychopharmacol, V11, P315

[2] SUBNEURONAL FATE OF INTRACEREBROVENTRICULAR INJECTED H-3 MELATONIN [J].

ANTONTAY, F ;

FORRAY, C ;

ORTEGACORONA, BG .

JOURNAL OF PINEAL RESEARCH, 1988, 5 (02) :125-133

[3] MELATONIN EFFECTS ON THE CYTOSKELETAL ORGANIZATION OF MDCK AND NEUROBLASTOMA N1E-115 CELLS [J].

BENITEZKING, G ;

HUERTODELGADILLO, L ;

ANTONTAY, F .

JOURNAL OF PINEAL RESEARCH, 1990, 9 (03) :209-220

[4] MELATONIN - A MAMMALIAN PINEAL HORMONE [J].

CARDINALI, DP .

ENDOCRINE REVIEWS, 1981, 2 (03) :327-346

[5] REGULATION OF INTRACELLULAR LEVELS OF CALMODULIN AND TUBULIN IN NORMAL AND TRANSFORMED-CELLS [J].

CHAFOULEAS, JG ;

PARDUE, RL ;

BRINKLEY, BR ;

DEDMAN, JR ;

MEANS, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (02) :996-1000

[6]

CHAFOULEAS JG, 1979, J BIOL CHEM, V254, P262

[7] EVIDENCE FOR A CYTOPLASMIC MELATONIN RECEPTOR [J].

COHEN, M ;

ROSELLE, D ;

CHABNER, B ;

SCHMIDT, TJ ;

LIPPMAN, M .

NATURE, 1978, 274 (5674) :894-895

[8] CHARACTERISTICS AND AUTORADIOGRAPHIC LOCALIZATION OF 2-[I-125]IODOMELATONIN BINDING-SITES IN DJUNGARIAN HAMSTER BRAIN [J].

DUNCAN, MJ ;

TAKAHASHI, JS ;

DUBOCOVICH, ML .

ENDOCRINOLOGY, 1989, 125 (02) :1011-1018

[9]

GREGORI L, 1985, J BIOL CHEM, V260, P5232

[10]

KLEIN DC, 1985, CIBA F SYMP, V117, P38

← 1 2 →