ISOLATION AND CHARACTERIZATION OF MUTANTS OF BACILLUS-THURINGIENSIS VAR ISRAELENSIS

被引：6

作者：

KIM, HS

COTE, JC

FRECHETTE, S

CHUNG, YS

机构：

[1] AGR CANADA,RECH STN,ST JEAN RICHELLEU J3B 3E6,PQ,CANADA

[2] UNIV MONTREAL,DEPT SCI BIOL,MONTREAL H3C 3J7,PQ,CANADA

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1994年 / 76卷 / 03期

关键词：

D O I：

10.1111/j.1365-2672.1994.tb01621.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several mutants of Bacillus thuringiensis var. israelensis HD-500 (Bti) were obtained after treatment with N-methyl-n'-nitro-N-nitrosoguanidine. On the basis of the production (+) or absence (-) of spore (Spo) and crystal (Cry) the strains were grouped into three categories: Spo + Cry +, Spo + Cry - and Spo - Cry + .NGI-22, a Spo + Cry - mutant lacked all crystal proteins. Both NGI-23 and NGI-23-1 were Spo - Cry + . NGI-23-1, however, produced multiple crystals per cell. These mutants could prove useful not only for analysing sporulation and crystal formation as well as the linkage between the two genetic processes but also for improving the potential of Bti as a microbial insecticide.

引用

页码：234 / 239

页数：6

共 27 条

[1] OPTIMAL CONDITIONS FOR MUTAGENESIS BY N-METHYL-N'-NITRO-N-NITROSOGUANIDINE IN ESCHERICHIA COLI K12 [J].