HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF NUCLEIC-ACIDS ON A FLUOROCARBON-BONDED SILICA-GEL COLUMN

被引：16

作者：

ITOH, H ^{[1
]}

KINOSHITA, T ^{[1
]}

NIMURA, N ^{[1
]}

机构：

[1] KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 662卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)85299-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of nucleic acids and related compounds was investigated using high-performance liquid chromatography on a new fluorocarbon-bonded silica gel column. The polyadenylate enzymic partial hydrolysate sample and a mixture of various polynucleotide samples were sufficiently separated in the reversed-phase mode using gradient elution with aqueous ammonium acetate-acetonitrile. A mixed-mode separation on the fluorinated phase coated with a tetraalkylammonium salt was also examined for the separation of various polynucleotides, including tRNAs.

引用

页码：95 / 99

页数：5

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