MICROSOMAL ETHANOL-OXIDIZING SYSTEM IN EUGLENA-GRACILIS - SIMILARITIES BETWEEN EUGLENA AND MAMMALIAN-CELL SYSTEMS

被引：10

作者：

JULISTIONO, H ^{[1
]}

BRIAND, J ^{[1
]}

机构：

[1] UNIV PARIS 07,MEMBRANES BIOL LAB,TOUR 54-53,2 PL JUSSIEU,F-75221 PARIS 05,FRANCE

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1992年 / 102卷 / 04期

关键词：

D O I：

10.1016/0305-0491(92)90074-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. ADH activity of Euglena grown with 50 mM ethanol decreased, but MEOS activity increased with a corresponding increase in the total amount of cytochrome P-450. 2. Phenobarbital treatment increased the total amount of cytochrome P-450. 3. CO and KCN, cytochrome P-450 ligands, diminished acetaldehyde formed from ethanol oxidation by MEOS. 4. The amounts of NAD(P)H cytochrome c reductases and cytochrome b5 type, components of microsomal monooxygenase reaction, have been spectrophotometrically measured. 5. NAD(P)H cytochrome c reductases activities were induced by phenobarbital. 6. DMSO, an inhibitor of rabbit MEOS, inhibited O2 consumption (11-20%) by Euglena grown with an ethanol, but not a lactate medium. 7. These studies indicate the presence of cytochrome P-450-dependent MEOS in Euglena similar to that in the mammalian hepatic cell.

引用

页码：747 / 755

页数：9

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